Engineered TALENs for Endogenous Human Gene Targets
The Joung Lab recently used their high-throughput FLASH method to construct a large series of engineered TALENs targeted to human genes involved in cancer and/or epigenetic regulation ( Reyon & Tsai et al., Nat Biotechnol. 2012). The table below lists 84 pairs of these TALENs that were shown to be active at their target sites in cultured U2OS cells.
| Target Gene Name | TALENs | Full Target Site (5' to 3'; half-sites in CAPS, spacer in lowercase) |
|---|---|---|
| ABL1 | TAL2380 & TAL2381 | TACCTATTATTACTTTATggggcagcagcctggaaAAGTACTTGGGGACCAA |
| AKT2 | TAL2382 & TAL2383 | TGTGTCTTGGGATGAGTGggtcagtgttctggtgCTCACAGGATGGCTGGCA |
| ALK | TAL2292 & TAL2293 | TCCTGTGGCTCCTGCCGCtgctgctttccacggcAGCTGTGGGCTCCGGGA |
| APC | TAL2294 & TAL2295 | TATGTACGCCTCCCTGGGctcgggtccggtcgccCCTTTGCCCGCTTCTGTA |
| ATM | TAL2402 & TAL2403 | TGAATTGGGATGCTGTTTttaggtattctattcaaaTTTATTTTACTGTCTTTA |
| AXIN2 | TAL2200 & TAL2201 | TCCCTCACCATGAGTAGCgctatgttggtgacttGCCTCCCGGACCCCAGCA |
| BAX | TAL2296 & TAL2297 | TGTGCGATCTCCAAGCACtgaggggcagaaactcCCGGATCGGGCGCTGCCA |
| BCL6 | TAL2412 & TAL2413 | TTTTCAAGTGAAGACAAAatggcctcgccggctgACAGCTGTATCCAGTTCA |
| BMPR1A | TAL2260 & TAL2261 | TACAATTGAACAATGCCTcagctatacatttacatCAGATTATTGGGAGCCTA |
| BRCA1 | TAL2384 & TAL2385 | TCCGAAGCTGACAGATGGgtattctttgacggggGGTAGGGGCGGAACCTGA |
| BRCA2 | TAL2302 & TAL2303 | TTAGACTTAGGTAAGTAAtgcaatatggtagactGGGGAGAACTACAAACTA |
| CBX3 | TAL2358 & TAL2359 | TCTGCAATAAAAAATGGCctccaacaaaactacaTTGGTAAGTTAATGAAAA |
| CBX8 | TAL2360 & TAL2361 | TGGAGCTTTCAGCGGTGGgggagcgggtgttcgcgGCCGAAGCCCTCCTGAA |
| CCND1 | TAL2304 & TAL2473 | TGGAACACCAGCTCCTGTgctgcgaagtggaaaccatCCGCCGCGCGTACCCCGA |
| CDC73 | TAL2202 & TAL2203 | TGCTTAGCGTCCTGCGACagtacaacatccagaaGAAGGAGATTGTGGTGAA |
| CDK4 | TAL2404 & TAL2405 | TCCCTTGATCTGAGAAtggctacctctcgataTGAGCCAGTGGCTGAAA |
| CHD4 | TAL2236 & TAL2237 | TGGCGTCGGGCCTGGGCtccccgtccccctgctcGGCGGGCAGTGAGGAGGA |
| CHD7 | TAL2238 & TAL2239 | TGTGTTGGAAGAAGATGGcagatccaggaatgatGAGTCTTTTTGGCGAGGA |
| CTNNB1 | TAL2262 & TAL2263 | TCCAGCGTGGACAATGGctactcaaggtttgtgTCATTAAATCTTTAGTTA |
| CYLD | TAL2386 & TAL2387 | TAATATCACAATGAGTTCaggcttatggagccaagaAAAAGTCACTTCACCCTA |
| DDB2 | TAL2282 & TAL2283 | TCACACGGAGGACGCGatggctcccaagaaacGCCCAGAAACCCAGAAGA |
| ERCC2 | TAL2306 & TAL2307 | TCCGGCCGGCGCCATGAagtgagaagggggctgGGGGTCGCGCTCGCTA |
| EWSR1 | TAL2252 & TAL2253 | TGGCGTCCACGGGTGAGTatggtggaactgcggtcGCGCCGGCGGTAGCCGGA |
| EXT1 | TAL2414 & TAL2415 | TGACCCAGGCAGGACACAtgcaggccaaaaaacgcTATTTCATCCTGCTCTCA |
| EXT2 | TAL2204 & TAL2205 | TTCCTCCCAGGGGGATGTcctgcgcctcagggtcCGGTGGTGGCCTGCGGCA |
| EZH2 | TAL2362 & TAL2363 | TGCTTTTAGAATAATCATgggccagactgggaagAAATCTGAGAAGGGACCA |
| FANCA | TAL2416 & TAL2417 | TAGGCGCCAAGGCCATGTccgactcgtgggtcccGAACTCCGCCTCGGGCCA |
| FANCC | TAL2254 & TAL2255 | TGAAGGGACATCACCTTTtcgctttttccaagatgGCTCAAGATTCAGTAGA |
| FANCF | TAL2314 & TAL2315 | TTCGCGCACCTCATGGaatcccttctgcagcaCCTGGATCGCTTTTCCGA |
| FANCG | TAL2288 & TAL2289 | TCGGCCACCATGTCCCgccagaccacctctgtGGGCTCCAGCTGCCTGGA |
| FES | TAL2390 & TAL2391 | TCCCCAGAACAGCACTATgggcttctcttccgagctGTGCAGCCCCCAGGGCCA |
| FGFR1 | TAL2316 & TAL2317 | TCTGCTCCCCACCGAGGAcctctgcatgcaggcaTGAATCCCAGGAGCCTA |
| FH | TAL2266 & TAL2267 | TGTACCGAGCACTTCGGCtcctcgcgcgctcgcgtCCCCTCGTGCGGGCTCCA |
| FLCN | TAL2206 & TAL2207 | TCTCCAAGGCACCATGAAtgccatcgtggctctctgCCACTTCTGCGAGCTCCA |
| FLT4 | TAL2320 & TAL2321 | TGCAGCGGGGCGCCGCGCtgtgcctgcgactgtggctCTGCCTGGGACTCCTGGA |
| FOXO1 | TAL2322 & TAL2323 | TCACCATGGCCGAGGCGcctcaggtggtggagaTCGACCCGGACTTCGA |
| FOXO3 | TAL2268 & TAL2269 | TCTCCGCTCGAAGTGGAGctggacccggagttcgagCCCCAGAGCCGTCCGCGA |
| GLI1 | TAL2270 & TAL2271 | TCCTCTGAGACGCCATGTtcaactcgatgaccccACCACCAATCAGTAGCTA |
| HDAC1 | TAL2364 & TAL2365 | TGGCGCAGACGCAGGGCacccggaggaaagtctgTTACTACTACGACGGTGA |
| HDAC2 | TAL2430 & TAL2431 | TGCGCTCACCTCCCTGCGgcctcctgaggtggtttgGTGGCCCCCTCCTCGCGA |
| HDAC6 | TAL2400 & TAL2401 | TCCTCAACTATGACCTCAaccggccaggattccaCCACAACCAGGCAGCGAA |
| HMGA2 | TAL2450 & TAL2451 | TGAGCGCACGCGGTGAGGgcgcggggcagccgtcCACTTCAGCCCAGGGACA |
| HOXA13 | TAL2208 & TAL2209 | TCCGTGCTCCTCCACCCCcgctggatcgagcccacCGTCATGTTTCTCTACGA |
| HOXA9 | TAL2444 & TAL2445 | TGGGCACGGTGATGGCcaccactggggccctgGGCAACTACTACGTGGA |
| HOXC13 | TAL2286 & TAL2287 | TCCAGCAGATCATGTCATgacgacttcgctgctcctGCATCCACGCTGGCCGGA |
| JAK2 | TAL2406 & TAL2407 | TCTGAAAAAGACTCTGCAtgggaatggcctgcctTACGATGACAGAAATGGA |
| KRAS | TAL2272 & TAL2273 | TGAAAATGACTGAATATAaacttgtggtagttggaGCTGGTGGCGTAGGCAA |
| MAP2K4 | TAL2324 & TAL2325 | TAGGGTCCCCGGCGCCAGgccacccggccgtcagCAGCATGCAGGGTAAGGA |
| MDM2 | TAL2274 & TAL2275 | TCCAAGCGCGAAAACCCCggatggtgaggagcaggTACTGGCCCGGCAGCGA |
| MET | TAL2326 & TAL2327 | TTATTATTACATGGCTTTgccttactgaggcttcATCTTGTCCTCTGGTCCA |
| MLH1 | TAL2328 & TAL2329 | TCTGGCGCCAAAATGTCGttcgtggcaggggttaTTCGGCGGCTGGACGAGA |
| MSH2 | TAL2330 & TAL2331 | TGAGGAGGTTTCGACATGgcggtgcagccgaaggAGACGCTGCAGTTGGAGA |
| MUTYH | TAL2218 & TAL2219 | TCACTGTCGGCGGCCATGacaccgctcgtctcccgcCTGAGTCGTCTGTGGGTA |
| MYC | TAL2398 & TAL2399 | TGCTTAGACGCTGGATTTttttcgggtagtggaaAACCAGGTAAGCACCGAA |
| MYCL1 | TAL2280 & TAL2281 | TCCCGCAGGGAGCGGACAtggactacgactcgtaCCAGCACTATTTCTACGA |
| MYCN | TAL2278 & TAL2279 | TGCCGAGCTGCTCCACgtccaccatgccgggcATGATCTGCAAGAACCCA |
| NBN | TAL2408 & TAL2409 | TGAGGAGCCGGACCGAtgtggaaactgctgccCGCCGCGGGCCCGGCA |
| NCOR1 | TAL2368 & TAL2369 | TCTTTACTGATAATGTCAagttcaggttatcctcCCAACCAAGGAGCATTCA |
| NCOR2 | TAL2284 & TAL2285 | TGGAGGGCCACTGAGCcccgctacccgccccaCAGCCTTTCCTACCCA |
| PDGFRA | TAL2340 & TAL2341 | TTGCGCTCGGGGCGGCCAtgtcggccggcgaggtCGAGCGCCTAGTGTCGGA |
| PDGFRB | TAL2448 & TAL2449 | TCTGCAGGACACCATGCGgcttccgggtgcgatgCCAGCTCTGGCCCTCAAA |
| PHF8 | TAL2246 & TAL2247 | TGAGTACTCCGCCTCTACcccggctgaagcccgcCCCCGCCGCCACCTATTA |
| PMS2 | TAL2418 & TAL2419 | TCGGGTGTTGCATCCATGgagcgagctgagagctcgAGGTGAGCGGGGCTCGCA |
| PTCH1 | TAL2342 & TAL2343 | TGGAACTGCTTAATAGaaacaggcttgtaattGTGAGTCCGCGCTGCA |
| PTEN | TAL2344 & TAL2345 | TCCCAGACATGACAGCCatcatcaaagagatcgTTAGCAGAAACAAAAGGA |
| RARA | TAL2420 & TAL2421 | TGGCATGGCCAGCAACAGcagctcctgcccgacacCTGGGGGCGGGCACCTCA |
| RBBP5 | TAL2370 & TAL2371 | TGCTGGGTGAGAAGGGCtgtggctgcgttttagaGAAGCGTTGGGTACTGGA |
| RECQL4 | TAL2220 & TAL2221 | TGCGGGACGTGCGGGAGCggctgcaggcgtgggaGCGCGCGTTCCGACGGCA |
| RET | TAL2346 & TAL2347 | TGAGTTCTGCCGGCCGCCggctcccgcaggggccaGGGCGAAGTTGGCGCCGA |
| RUNX1 | TAL2452 & TAL2453 | TTCAGGAGGAAGCGATGGcttcagacagcatattTGAGTCATTTCCTTCGTA |
| SDHB | TAL2222 & TAL2223 | TCTCCTTGAGGCGCCGGTtgccggccacaaccctTGGCGGAGCCTGCCTGCA |
| SDHC | TAL2228 & TAL2229 | TGTTGCTGAGGTGACTTCagtgggactgggagttggtGCCTGCGGCCCTCCGGA |
| SDHD | TAL2230 & TAL2231 | TCAGGAACGAGATGGCGGttctctggaggctgagtGCCGTTTGCGGTGCCCTA |
| SETDB1 | TAL2374 & TAL2375 | TGCAGAGGACAAAAGCATgtcttcccttcctgggTGCATTGGTTTGGATGCA |
| SIRT6 | TAL2454 & TAL2455 | TTACGCGGCGGGGCTGTCgccgtacgcggacaagggCAAGTGCGGCCTCCCGGA |
| SMAD2 | TAL2422 & TAL2423 | TTTGGTAAGAACATGTCGtccatcttgccattcacGCCGCCAGTTGTGAAGA |
| SS18 | TAL2442 & TAL2443 | TGGTGACGGCGGCAACATgtctgtggctttcgcggCCCCGAGGCAGCGAGGCA |
| SUZ12 | TAL2376 & TAL2377 | TGGCGCCTCAGAAGCAcggcggtgggggagggGGCGGCTCGGGGCCCA |
| TFE3 | TAL2424 & TAL2425 | TCATGTCTCATGCGGCCGaaccagctcgggatggCGTAGAGGCCAGCGCGGA |
| TP53 | TAL2348 & TAL2472 | TTGCCGTCCCAAGCAATGgatgatttgatgctgtcCCCGGACGATATTGAACA |
| TSC2 | TAL2396 & TAL2397 | TCCTGGTCCACCATGGCcaaaccaacaagcaaagATTCAGGCTTGAAGGAGA |
| VHL | TAL2456 & TAL2457 | TCTGGATCGCGGAGGGAAtgccccggagggcggaGAACTGGGACGAGGCCGA |
| XPA | TAL2458 & TAL2459 | TGGGCCAGAGATGGCGGCggccgacggggctttgCCGGAGGCGGCGGCTTTA |
| XPC | TAL2350 & TAL2351 | TGCCCAGACAAGCAACATggctcggaaacgcgcggccGGCGGGGAGCCGCGGGGA |