TALEN Kit
(Kit #
1000000038
)
Depositing Lab: Stephen Ekker
This kit contains all 256 possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs.
This kit consists of three 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs.
Original Publication
High Efficiency In Vivo Genome Engineering With a Simplified 15-RVD GoldyTALEN Design. Ma AC, Lee HB, Clark KJ and Ekker SC. PLoS One 2013 May 29;8(5):e65259. doi: 10.1371/journal.pone.0065259. PubMed PMID 23734242.
Description
This Complete pFUS_B4 Collection contains all 256 possible combinations of pFUS_B4 clones built with TALE repeat modules contain RVDs NI, HD, NN and NG. The collection is aimed to work in complement with the Golden Gate TALEN and TAL Effector Kit 2.0 to assemble highly active 15-RVD GoldyTALENs without the need for building individual pFUS_B4 intermediates.
Besides the RCIscript-GoldyTALEN backbone which is highly active in zebrafish, the collection is also compatible with other Golden Gate TALEN backbones.
Kit Documentation
Ekker Lab TALEN Kit 1.3Â MBProtocol
Download a protocol for synthesizing 15-RVD TALENs:
Ekker lab protocol 60.5Â KBHow to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The complete pFUS_B4 collection for Golden Gate assembly of 15-RVD TALENs was a gift from Stephen Ekker (Addgene kit # 1000000038)"
For your Reference section:
High Efficiency In Vivo Genome Engineering With a Simplified 15-RVD GoldyTALEN Design. Ma AC, Lee HB, Clark KJ and Ekker SC. PLoS One 2013 May 29;8(5):e65259. doi: 10.1371/journal.pone.0065259. PubMed PMID 23734242.
Ekker Lab TALEN kit - #1000000038
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Spectinomycin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| Plate 1 / A / 1 | pFUSB4_AAAA | Spectinomycin |
| Plate 1 / A / 2 | pFUSB4_AAGA | Spectinomycin |
| Plate 1 / A / 3 | pFUSB4_ACAA | Spectinomycin |
| Plate 1 / A / 4 | pFUSB4_ACGA | Spectinomycin |
| Plate 1 / A / 5 | pFUSB4_AGAA | Spectinomycin |
| Plate 1 / A / 6 | pFUSB4_AGGA | Spectinomycin |
| Plate 1 / A / 7 | pFUSB4_ATAA | Spectinomycin |
| Plate 1 / A / 8 | pFUSB4_ATGA | Spectinomycin |
| Plate 1 / A / 9 | pFUSB4_CAAA | Spectinomycin |
| Plate 1 / A / 10 | pFUSB4_CAGA | Spectinomycin |
| Plate 1 / A / 11 | pFUSB4_CCAA | Spectinomycin |
| Plate 1 / A / 12 | pFUSB4_CCGA | Spectinomycin |
| Plate 1 / B / 1 | pFUSB4_AAAC | Spectinomycin |
| Plate 1 / B / 2 | pFUSB4_AAGC | Spectinomycin |
| Plate 1 / B / 3 | pFUSB4_ACAC | Spectinomycin |
| Plate 1 / B / 4 | pFUSB4_ACGC | Spectinomycin |
| Plate 1 / B / 5 | pFUSB4_AGAC | Spectinomycin |
| Plate 1 / B / 6 | pFUSB4_AGGC | Spectinomycin |
| Plate 1 / B / 7 | pFUSB4_ATAC | Spectinomycin |
| Plate 1 / B / 8 | pFUSB4_ATGC | Spectinomycin |
| Plate 1 / B / 9 | pFUSB4_CAAC | Spectinomycin |
| Plate 1 / B / 10 | pFUSB4_CAGC | Spectinomycin |
| Plate 1 / B / 11 | pFUSB4_CCAC | Spectinomycin |
| Plate 1 / B / 12 | pFUSB4_CCGC | Spectinomycin |
| Plate 1 / C / 1 | pFUSB4_AAAG | Spectinomycin |
| Plate 1 / C / 2 | pFUSB4_AAGG | Spectinomycin |
| Plate 1 / C / 3 | pFUSB4_ACAG | Spectinomycin |
| Plate 1 / C / 4 | pFUSB4_ACGG | Spectinomycin |
| Plate 1 / C / 5 | pFUSB4_AGAG | Spectinomycin |
| Plate 1 / C / 6 | pFUSB4_AGGG | Spectinomycin |
| Plate 1 / C / 7 | pFUSB4_ATAG | Spectinomycin |
| Plate 1 / C / 8 | pFUSB4_ATGG | Spectinomycin |
| Plate 1 / C / 9 | pFUSB4_CAAG | Spectinomycin |
| Plate 1 / C / 10 | pFUSB4_CAGG | Spectinomycin |
| Plate 1 / C / 11 | pFUSB4_CCAG | Spectinomycin |
| Plate 1 / C / 12 | pFUSB4_CCGG | Spectinomycin |
| Plate 1 / D / 1 | pFUSB4_AAAT | Spectinomycin |
| Plate 1 / D / 2 | pFUSB4_AAGT | Spectinomycin |
| Plate 1 / D / 3 | pFUSB4_ACAT | Spectinomycin |
| Plate 1 / D / 4 | pFUSB4_ACGT | Spectinomycin |
| Plate 1 / D / 5 | pFUSB4_AGAT | Spectinomycin |
| Plate 1 / D / 6 | pFUSB4_AGGT | Spectinomycin |
| Plate 1 / D / 7 | pFUSB4_ATAT | Spectinomycin |
| Plate 1 / D / 8 | pFUSB4_ATGT | Spectinomycin |
| Plate 1 / D / 9 | pFUSB4_CAAT | Spectinomycin |
| Plate 1 / D / 10 | pFUSB4_CAGT | Spectinomycin |
| Plate 1 / D / 11 | pFUSB4_CCAT | Spectinomycin |
| Plate 1 / D / 12 | pFUSB4_CCGT | Spectinomycin |
| Plate 1 / E / 1 | pFUSB4_AACA | Spectinomycin |
| Plate 1 / E / 2 | pFUSB4_AATA | Spectinomycin |
| Plate 1 / E / 3 | pFUSB4_ACCA | Spectinomycin |
| Plate 1 / E / 4 | pFUSB4_ACTA | Spectinomycin |
| Plate 1 / E / 5 | pFUSB4_AGCA | Spectinomycin |
| Plate 1 / E / 6 | pFUSB4_AGTA | Spectinomycin |
| Plate 1 / E / 7 | pFUSB4_ATCA | Spectinomycin |
| Plate 1 / E / 8 | pFUSB4_ATTA | Spectinomycin |
| Plate 1 / E / 9 | pFUSB4_CACA | Spectinomycin |
| Plate 1 / E / 10 | pFUSB4_CATA | Spectinomycin |
| Plate 1 / E / 11 | pFUSB4_CCCA | Spectinomycin |
| Plate 1 / E / 12 | pFUSB4_CCTA | Spectinomycin |
| Plate 1 / F / 1 | pFUSB4_AACC | Spectinomycin |
| Plate 1 / F / 2 | pFUSB4_AATC | Spectinomycin |
| Plate 1 / F / 3 | pFUSB4_ACCC | Spectinomycin |
| Plate 1 / F / 4 | pFUSB4_ACTC | Spectinomycin |
| Plate 1 / F / 5 | pFUSB4_AGCC | Spectinomycin |
| Plate 1 / F / 6 | pFUSB4_AGTC | Spectinomycin |
| Plate 1 / F / 7 | pFUSB4_ATCC | Spectinomycin |
| Plate 1 / F / 8 | pFUSB4_ATTC | Spectinomycin |
| Plate 1 / F / 9 | pFUSB4_CACC | Spectinomycin |
| Plate 1 / F / 10 | pFUSB4_CATC | Spectinomycin |
| Plate 1 / F / 11 | pFUSB4_CCCC | Spectinomycin |
| Plate 1 / F / 12 | pFUSB4_CCTC | Spectinomycin |
| Plate 1 / G / 1 | pFUSB4_AACG | Spectinomycin |
| Plate 1 / G / 2 | pFUSB4_AATG | Spectinomycin |
| Plate 1 / G / 3 | pFUSB4_ACCG | Spectinomycin |
| Plate 1 / G / 4 | pFUSB4_ACTG | Spectinomycin |
| Plate 1 / G / 5 | pFUSB4_AGCG | Spectinomycin |
| Plate 1 / G / 6 | pFUSB4_AGTG | Spectinomycin |
| Plate 1 / G / 7 | pFUSB4_ATCG | Spectinomycin |
| Plate 1 / G / 8 | pFUSB4_ATTG | Spectinomycin |
| Plate 1 / G / 9 | pFUSB4_CACG | Spectinomycin |
| Plate 1 / G / 10 | pFUSB4_CATG | Spectinomycin |
| Plate 1 / G / 11 | pFUSB4_CCCG | Spectinomycin |
| Plate 1 / G / 12 | pFUSB4_CCTG | Spectinomycin |
| Plate 1 / H / 1 | pFUSB4_AACT | Spectinomycin |
| Plate 1 / H / 2 | pFUSB4_AATT | Spectinomycin |
| Plate 1 / H / 3 | pFUSB4_ACCT | Spectinomycin |
| Plate 1 / H / 4 | pFUSB4_ACTT | Spectinomycin |
| Plate 1 / H / 5 | pFUSB4_AGCT | Spectinomycin |
| Plate 1 / H / 6 | pFUSB4_AGTT | Spectinomycin |
| Plate 1 / H / 7 | pFUSB4_ATCT | Spectinomycin |
| Plate 1 / H / 8 | pFUSB4_ATTT | Spectinomycin |
| Plate 1 / H / 9 | pFUSB4_CACT | Spectinomycin |
| Plate 1 / H / 10 | pFUSB4_CATT | Spectinomycin |
| Plate 1 / H / 11 | pFUSB4_CCCT | Spectinomycin |
| Plate 1 / H / 12 | pFUSB4_CCTT | Spectinomycin |
| Plate 2 / A / 1 | pFUSB4_CGAA | Spectinomycin |
| Plate 2 / A / 2 | pFUSB4_CGGA | Spectinomycin |
| Plate 2 / A / 3 | pFUSB4_CTAA | Spectinomycin |
| Plate 2 / A / 4 | pFUSB4_CTGA | Spectinomycin |
| Plate 2 / A / 5 | pFUSB4_GAAA | Spectinomycin |
| Plate 2 / A / 6 | pFUSB4_GAGA | Spectinomycin |
| Plate 2 / A / 7 | pFUSB4_GCAA | Spectinomycin |
| Plate 2 / A / 8 | pFUSB4_GCGA | Spectinomycin |
| Plate 2 / A / 9 | pFUSB4_GGAA | Spectinomycin |
| Plate 2 / A / 10 | pFUSB4_GGGA | Spectinomycin |
| Plate 2 / A / 11 | pFUSB4_GTAA | Spectinomycin |
| Plate 2 / A / 12 | pFUSB4_GTGA | Spectinomycin |
| Plate 2 / B / 1 | pFUSB4_CGAC | Spectinomycin |
| Plate 2 / B / 2 | pFUSB4_CGGC | Spectinomycin |
| Plate 2 / B / 3 | pFUSB4_CTAC | Spectinomycin |
| Plate 2 / B / 4 | pFUSB4_CTGC | Spectinomycin |
| Plate 2 / B / 5 | pFUSB4_GAAC | Spectinomycin |
| Plate 2 / B / 6 | pFUSB4_GAGC | Spectinomycin |
| Plate 2 / B / 7 | pFUSB4_GCAC | Spectinomycin |
| Plate 2 / B / 8 | pFUSB4_GCGC | Spectinomycin |
| Plate 2 / B / 9 | pFUSB4_GGAC | Spectinomycin |
| Plate 2 / B / 10 | pFUSB4_GGGC | Spectinomycin |
| Plate 2 / B / 11 | pFUSB4_GTAC | Spectinomycin |
| Plate 2 / B / 12 | pFUSB4_GTGC | Spectinomycin |
| Plate 2 / C / 1 | pFUSB4_CGAG | Spectinomycin |
| Plate 2 / C / 2 | pFUSB4_CGGG | Spectinomycin |
| Plate 2 / C / 3 | pFUSB4_CTAG | Spectinomycin |
| Plate 2 / C / 4 | pFUSB4_CTGG | Spectinomycin |
| Plate 2 / C / 5 | pFUSB4_GAAG | Spectinomycin |
| Plate 2 / C / 6 | pFUSB4_GAGG | Spectinomycin |
| Plate 2 / C / 7 | pFUSB4_GCAG | Spectinomycin |
| Plate 2 / C / 8 | pFUSB4_GCGG | Spectinomycin |
| Plate 2 / C / 9 | pFUSB4_GGAG | Spectinomycin |
| Plate 2 / C / 10 | pFUSB4_GGGG | Spectinomycin |
| Plate 2 / C / 11 | pFUSB4_GTAG | Spectinomycin |
| Plate 2 / C / 12 | pFUSB4_GTGG | Spectinomycin |
| Plate 2 / D / 1 | pFUSB4_CGAT | Spectinomycin |
| Plate 2 / D / 2 | pFUSB4_CGGT | Spectinomycin |
| Plate 2 / D / 3 | pFUSB4_CTAT | Spectinomycin |
| Plate 2 / D / 4 | pFUSB4_CTGT | Spectinomycin |
| Plate 2 / D / 5 | pFUSB4_GAAT | Spectinomycin |
| Plate 2 / D / 6 | pFUSB4_GAGT | Spectinomycin |
| Plate 2 / D / 7 | pFUSB4_GCAT | Spectinomycin |
| Plate 2 / D / 8 | pFUSB4_GCGT | Spectinomycin |
| Plate 2 / D / 9 | pFUSB4_GGAT | Spectinomycin |
| Plate 2 / D / 10 | pFUSB4_GGGT | Spectinomycin |
| Plate 2 / D / 11 | pFUSB4_GTAT | Spectinomycin |
| Plate 2 / D / 12 | pFUSB4_GTGT | Spectinomycin |
| Plate 2 / E / 1 | pFUSB4_CGCA | Spectinomycin |
| Plate 2 / E / 2 | pFUSB4_CGTA | Spectinomycin |
| Plate 2 / E / 3 | pFUSB4_CTCA | Spectinomycin |
| Plate 2 / E / 4 | pFUSB4_CTTA | Spectinomycin |
| Plate 2 / E / 5 | pFUSB4_GACA | Spectinomycin |
| Plate 2 / E / 6 | pFUSB4_GATA | Spectinomycin |
| Plate 2 / E / 7 | pFUSB4_GCCA | Spectinomycin |
| Plate 2 / E / 8 | pFUSB4_GCTA | Spectinomycin |
| Plate 2 / E / 9 | pFUSB4_GGCA | Spectinomycin |
| Plate 2 / E / 10 | pFUSB4_GGTA | Spectinomycin |
| Plate 2 / E / 11 | pFUSB4_GTCA | Spectinomycin |
| Plate 2 / E / 12 | pFUSB4_GTTA | Spectinomycin |
| Plate 2 / F / 1 | pFUSB4_CGCC | Spectinomycin |
| Plate 2 / F / 2 | pFUSB4_CGTC | Spectinomycin |
| Plate 2 / F / 3 | pFUSB4_CTCC | Spectinomycin |
| Plate 2 / F / 4 | pFUSB4_CTTC | Spectinomycin |
| Plate 2 / F / 5 | pFUSB4_GACC | Spectinomycin |
| Plate 2 / F / 6 | pFUSB4_GATC | Spectinomycin |
| Plate 2 / F / 7 | pFUSB4_GCCC | Spectinomycin |
| Plate 2 / F / 8 | pFUSB4_GCTC | Spectinomycin |
| Plate 2 / F / 9 | pFUSB4_GGCC | Spectinomycin |
| Plate 2 / F / 10 | pFUSB4_GGTC | Spectinomycin |
| Plate 2 / F / 11 | pFUSB4_GTCC | Spectinomycin |
| Plate 2 / F / 12 | pFUSB4_GTTC | Spectinomycin |
| Plate 2 / G / 1 | pFUSB4_CGCG | Spectinomycin |
| Plate 2 / G / 2 | pFUSB4_CGTG | Spectinomycin |
| Plate 2 / G / 3 | pFUSB4_CTCG | Spectinomycin |
| Plate 2 / G / 4 | pFUSB4_CTTG | Spectinomycin |
| Plate 2 / G / 5 | pFUSB4_GACG | Spectinomycin |
| Plate 2 / G / 6 | pFUSB4_GATG | Spectinomycin |
| Plate 2 / G / 7 | pFUSB4_GCCG | Spectinomycin |
| Plate 2 / G / 8 | pFUSB4_GCTG | Spectinomycin |
| Plate 2 / G / 9 | pFUSB4_GGCG | Spectinomycin |
| Plate 2 / G / 10 | pFUSB4_GGTG | Spectinomycin |
| Plate 2 / G / 11 | pFUSB4_GTCG | Spectinomycin |
| Plate 2 / G / 12 | pFUSB4_GTTG | Spectinomycin |
| Plate 2 / H / 1 | pFUSB4_CGCT | Spectinomycin |
| Plate 2 / H / 2 | pFUSB4_CGTT | Spectinomycin |
| Plate 2 / H / 3 | pFUSB4_CTCT | Spectinomycin |
| Plate 2 / H / 4 | pFUSB4_CTTT | Spectinomycin |
| Plate 2 / H / 5 | pFUSB4_GACT | Spectinomycin |
| Plate 2 / H / 6 | pFUSB4_GATT | Spectinomycin |
| Plate 2 / H / 7 | pFUSB4_GCCT | Spectinomycin |
| Plate 2 / H / 8 | pFUSB4_GCTT | Spectinomycin |
| Plate 2 / H / 9 | pFUSB4_GGCT | Spectinomycin |
| Plate 2 / H / 10 | pFUSB4_GGTT | Spectinomycin |
| Plate 2 / H / 11 | pFUSB4_GTCT | Spectinomycin |
| Plate 2 / H / 12 | pFUSB4_GTTT | Spectinomycin |
| Plate 3 / A / 1 | pFUSB4_TAAA | Spectinomycin |
| Plate 3 / A / 2 | pFUSB4_TAGA | Spectinomycin |
| Plate 3 / A / 3 | pFUSB4_TCAA | Spectinomycin |
| Plate 3 / A / 4 | pFUSB4_TCGA | Spectinomycin |
| Plate 3 / A / 5 | pFUSB4_TGAA | Spectinomycin |
| Plate 3 / A / 6 | pFUSB4_TGGA | Spectinomycin |
| Plate 3 / A / 7 | pFUSB4_TTAA | Spectinomycin |
| Plate 3 / A / 8 | pFUSB4_TTGA | Spectinomycin |
| Plate 3 / A / 9 | pFUSB4_TTTT | Spectinomycin |
| Plate 3 / A / 10 | pFUSB4_TTCT | Spectinomycin |
| Plate 3 / A / 11 | pFUSB4_TGTT | Spectinomycin |
| Plate 3 / A / 12 | pFUSB4_TGCT | Spectinomycin |
| Plate 3 / B / 1 | pFUSB4_TAAC | Spectinomycin |
| Plate 3 / B / 2 | pFUSB4_TAGC | Spectinomycin |
| Plate 3 / B / 3 | pFUSB4_TCAC | Spectinomycin |
| Plate 3 / B / 4 | pFUSB4_TCGC | Spectinomycin |
| Plate 3 / B / 5 | pFUSB4_TGAC | Spectinomycin |
| Plate 3 / B / 6 | pFUSB4_TGGC | Spectinomycin |
| Plate 3 / B / 7 | pFUSB4_TTAC | Spectinomycin |
| Plate 3 / B / 8 | pFUSB4_TTGC | Spectinomycin |
| Plate 3 / B / 9 | pFUSB4_TACT | Spectinomycin |
| Plate 3 / B / 10 | pFUSB4_TATT | Spectinomycin |
| Plate 3 / B / 11 | pFUSB4_TCCT | Spectinomycin |
| Plate 3 / B / 12 | pFUSB4_TCTT | Spectinomycin |
| Plate 3 / C / 1 | pFUSB4_TAAG | Spectinomycin |
| Plate 3 / C / 2 | pFUSB4_TAGG | Spectinomycin |
| Plate 3 / C / 3 | pFUSB4_TCAG | Spectinomycin |
| Plate 3 / C / 4 | pFUSB4_TCGG | Spectinomycin |
| Plate 3 / C / 5 | pFUSB4_TGAG | Spectinomycin |
| Plate 3 / C / 6 | pFUSB4_TGGG | Spectinomycin |
| Plate 3 / C / 7 | pFUSB4_TTAG | Spectinomycin |
| Plate 3 / C / 8 | pFUSB4_TTGG | Spectinomycin |
| Plate 3 / C / 9 | pFUSB4_TACG | Spectinomycin |
| Plate 3 / C / 10 | pFUSB4_TATG | Spectinomycin |
| Plate 3 / C / 11 | pFUSB4_TCCG | Spectinomycin |
| Plate 3 / C / 12 | pFUSB4_TCTG | Spectinomycin |
| Plate 3 / D / 1 | pFUSB4_TAAT | Spectinomycin |
| Plate 3 / D / 2 | pFUSB4_TAGT | Spectinomycin |
| Plate 3 / D / 3 | pFUSB4_TCAT | Spectinomycin |
| Plate 3 / D / 4 | pFUSB4_TCGT | Spectinomycin |
| Plate 3 / D / 5 | pFUSB4_TGAT | Spectinomycin |
| Plate 3 / D / 6 | pFUSB4_TGGT | Spectinomycin |
| Plate 3 / D / 7 | pFUSB4_TTAT | Spectinomycin |
| Plate 3 / D / 8 | pFUSB4_TTGT | Spectinomycin |
| Plate 3 / D / 9 | pFUSB4_TGCG | Spectinomycin |
| Plate 3 / D / 10 | pFUSB4_TGTG | Spectinomycin |
| Plate 3 / D / 11 | pFUSB4_TTCG | Spectinomycin |
| Plate 3 / D / 12 | pFUSB4_TTTG | Spectinomycin |
| Plate 3 / E / 1 | pFUSB4_TACA | Spectinomycin |
| Plate 3 / E / 2 | pFUSB4_TATA | Spectinomycin |
| Plate 3 / E / 3 | pFUSB4_TCCA | Spectinomycin |
| Plate 3 / E / 4 | pFUSB4_TCTA | Spectinomycin |
| Plate 3 / E / 5 | pFUSB4_TGCA | Spectinomycin |
| Plate 3 / E / 6 | pFUSB4_TGTA | Spectinomycin |
| Plate 3 / E / 7 | pFUSB4_TTCA | Spectinomycin |
| Plate 3 / E / 8 | pFUSB4_TTTA | Spectinomycin |
| Plate 3 / E / 9 | pFUSB4_TGCC | Spectinomycin |
| Plate 3 / E / 10 | pFUSB4_TGTC | Spectinomycin |
| Plate 3 / E / 11 | pFUSB4_TTCC | Spectinomycin |
| Plate 3 / E / 12 | pFUSB4_TTTC | Spectinomycin |
| Plate 3 / F / 1 | pFUSB4_TACC | Spectinomycin |
| Plate 3 / F / 2 | pFUSB4_TATC | Spectinomycin |
| Plate 3 / F / 3 | pFUSB4_TCCC | Spectinomycin |
| Plate 3 / F / 4 | pFUSB4_TCTC | Spectinomycin |
Kit Plate Map - #1000000038
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