...introduced, the puromycin resistance marker encoded in pLKO.1 allows for convenient stable selection. Figure ... drives expression of puromycin. Puro R Puromycin resistance gene for selection of pLKO.1 plasmid in mammalian...differently to puromycin selection. Addgene strongly recommends that you determine the optimal puromycin concentration...expression of the puromycin resistance gene. f. To select for infected cells, add puromycin to the media at...positive control for the puromycin selection. Days 4+: g. Change to fresh puromycin-containing media as needed... the optimal puromycin concentration F.3 Protocol for lentiviral infection and selection G. Safety H. ... target selection are continuously being improved. Below are suggestions for target selection. Use an ...

...adapted to a variety of target cell lines and selection markers. Note this assay requires staining resistant...were treated with puromycin. Colonies were allowed to grow in the presence of selective reagent for 12 days... 2: Replace media with fresh media containing selection reagent Days 3–14: Change media as needed Days...media exchanges every 3–4 days. After 12 days of selection, no cells have survived in the untransduced well...