...800 ng pooled library DNA (100 ng plasmid DNA per 25 µL electrocompetent cells) Less library DNA can be...Amplification CRISPR Library Amplification You may also like... Pooled libraries Guide to Using Pooled Libraries...All CRISPR Pooled Libraries Molecular Biology Reference How do I process my Addgene pooled library? Introduction...point for CRISPR libraries. This protocol allows the amplification of a pooled-plasmid library in Escherichia...amplification of CRISPR pooled plasmid libraries in Escherichia coli cells. Protocols...obtained the pooled library from Addgene, these protocols can be found on the Pooled Library page for the...efficiency uptake of plasmid library DNA. This quantity of cells is sufficient for libraries up to 200,000 individual...

...Watch the Video! CRISPR Library Amplification Amplify CRISPR pooled-plasmid libraries Diagnostic Restriction...the Video! DNA Ligation Assemble DNA using DNA ligase Bacterial Transformation Introduce DNA into a bacterial...phenol-chloroform extract, and precipitate DNA DNA Quantification Measure DNA concentration with a spectrophotometer...and analyze plasmid DNA with restriction enzymes Agarose Gel Electrophoresis Separate DNA by size on an...Add a short stretch of DNA to a plasmid Gibson Assembly Combine overlapping DNA fragments in a single ...complicated procedures. Plasmid Cloning Protocols for constructing and analyzing your plasmids. Virus Protocols...Store bacterial strains or plasmids for long term use Watch the Video! DNA Purification Miniprep, phenol-chloroform...