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  1. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral particles...Recommended Materials Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells... transfection: 1 μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid...

  2. Lentivirus Production

    Type
    Protocol
    ...into OptiPro SFM: Reagent Amount per 10 cm dish* psPAX2 1.3 pmol pMD2.G 0.72 pmol Transfer Plasmid* 1.64...
Showing: 1 - 2 of 2 results