...designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational...is for labs that work with pathogens including organisms such as Staphylococcus aureus or Vibrio cholerae...administrators, or anyone else associated with Addgene, can be held responsible for your use of the information...

... precipitated protein particles Bottom phase - Organic phase (protein) Pipet the aqueous DNA layer and...

...Molecular Biology Reference How do I process my Addgene pooled library? Introduction Please refer to our...depositor for a specific pooled library available at Addgene. We recommend following the depositor's specific...DNA: guide RNAs targeting different genes in an organism's genome, for example. Amplification is usually...results. If you obtained the pooled library from Addgene, these protocols can be found on the Pooled Library...with ice and water to create slurry) 100 mL LB At Addgene we use premixed LB (VWR 101414-072) for convenience...Help Center to get details on how to process your Addgene pooled library...