...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the...TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep DNA) 5 μL 10x NEB...TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning vector. The vector is 8901 base...Producing Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles...-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A.1 The ...TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing... pLKO derived vectors that may also be useful for your experiment. To see these vectors, visit Addgene...

...from a lentiviral vector transfected into 293T cells using a polyethylenimine (PEI) transfection protocol...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmids...the diluted DNA mixture. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate...Viral Vector Guides Mol Bio Protocols Introduction This protocol can be used to produce lentivirus from...possible range of ratios to test: Ratio of DNA:PEI μg of DNA μL of 1 mg/mL PEI 1:1 18.9 18.9 1:2 18.9 37.8...using μg total DNA to μg PEI ratios of 1:1, 1:2, 1:3 and 1:6. The 1:2 and 1:3 total DNA:PEI μg ratios provided...polyethersulfone (PES) filter Transfection grade DNAs Reagent Preparation DMEM Complete (also known as...

...aspirate supernatant. Extract genomic DNA according to the GeneJet Genomic DNA Purification Kit instructions...., the lentiviral particles are serially diluted and used to transduce HEK293T cells. Genomic DNA is extracted... of Lentivirus Vectors You may also like... Lentivirus Colony Formation Titration Assay Lentivirus Fluorescence... (ddPCR) for lentivirus vector titration. Protocols... Protocols ddPCR Titration of Lentivirus Vectors ddPCR Titration... use droplet digital PCR (ddPCR) to titer lentivirus vectors. This protocol specifically uses primers ...Thermo Scientific, 10199-452 Reagents GeneJet Genomic DNA Purification Kit, Thermo Fisher, K0721 6-well tissue...

... Separate DNA by size on an agarose gel Watch the Video! DNA Ligation Assemble DNA using DNA ligase Bacterial... the Video! DNA Purification Miniprep, phenol-chloroform extract, and precipitate DNA DNA Quantification...Measure DNA concentration with a spectrophotometer Restriction Digests Cut and analyze plasmid DNA with ...FAQ Watch the Video! Purifying DNA from an Agarose gel Isolate specific DNA bands cut from a gel Watch the...Add a short stretch of DNA to a plasmid Gibson Assembly Combine overlapping DNA fragments in a single ...polymerase pLKO.1 - TRC Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone used by the ... Introduce plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine...