...from a lentiviral vector transfected into 293T cells using a polyethylenimine (PEI) transfection protocol...Viral Vector Guides Mol Bio Protocols Introduction This protocol can be used to produce lentivirus from...determine what ratio gives the highest percentage of GFP positive cells. Refer to the table below for a possible...Data Figure 1: 293T cells were transfected with the GFP-expression plasmid pRosetta using μg total DNA to...efficiencies as measured by the highest proportion of GFP positive cells without limiting cell growth. Left...Left panels: bright field images; right panels: GFP channel images. Last reviewed on: August 2, 2023... Protocols Lentivirus Production Lentivirus Production You may also ...