Coomassie Purity Stain of Recombinant Antibodies
Type
Protocol
...Coomassie G-250 stain (Addgene uses SimplyBlue SafeStain) and ImageJ software. The sample is separated ...recombinant antibody, 0.9–1.1 mg/mL SimplyBlue SafeStain, Thermo Fisher, LC6060 4-12% NuPage Novex bis-...the water in the sink. Add 20 mL of SimplyBlue SafeStain and incubate for 1 h with gentle agitation on ...on a rocking platform. Pour off the SimplyBlue SafeStain in the sink. Add 100 mL of deionized water and...