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Addgene
Showing: 1 - 5 of 5 results
  1. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...Coomassie G-250 stain (Addgene uses SimplyBlue SafeStain) and ImageJ software. The sample is separated ...recombinant antibody, 0.9–1.1 mg/mL SimplyBlue SafeStain, Thermo Fisher, LC6060 4-12% NuPage Novex bis-...the water in the sink. Add 20 mL of SimplyBlue SafeStain and incubate for 1 h with gentle agitation on ...on a rocking platform. Pour off the SimplyBlue SafeStain in the sink. Add 100 mL of deionized water and...
  2. CRISPR Library Amplification

    Type
    Protocol
    ...taking place during electroporation. Arcing would manifest as a loud pop often accompanied by some light ...
  3. Lentivirus ddPCR Titration

    Type
    Protocol
    ...and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious Titer...
Showing: 1 - 5 of 5 results