Addgene

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Protocol - Bacterial Transformation

Type

Protocol

...BACs, what can I do? Chemically competent cells are fast and easy to use, but are less efficient at taking...
Handling Plasmids from Addgene - Purifying Plasmid DNA

Type

Protocol

... and FAQ Plasmid purification kits provide the fastest way to obtain a high concentration of clean plasmid...
AAV Production in HEK293 Cells

Type

Protocol

...of PEG. Stirring under medium heat will promote faster dissolution. Adjust the pH to ~7.4. Autoclave or...
CRISPR Library Amplification

Type

Protocol

... that contain a recombinant plasmid likely grow faster in the closed system of a crowded plate or culture...

Showing: 1 - 4 of 4 results