...amplification of CRISPR pooled plasmid libraries in Escherichia coli cells....starting point for CRISPR libraries. This protocol allows the amplification of a pooled-plasmid library in Escherichia...differences, and plasmid recombination can all impact the representation of individual plasmids in the pooled... ). Lentiviral plasmids can recombine between their LTRs resulting in a smaller plasmid containing only... Protocols CRISPR Library Amplification...Amplification CRISPR Library Amplification You may also like... Pooled libraries Guide to Using Pooled Libraries...Libraries All CRISPR Pooled Libraries Molecular Biology Reference How do I process my Addgene pooled library...

...Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions...Instructions for Storing and Handling Plasmids Genomic Deletions with CRISPR Generate genomic deletions in mammalian...mammalian cells lines using CRISPR/Cas9 Genomic Deletions with CRISPR Protocol Over-Agar Antibiotic Plating...Protocol Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Diagnostic Restriction...Transformations We walk you through a standard plasmid transformation protocol and offer some tips and...Center How to Deposit Your guide to depositing plasmids with Addgene Depositing Page Career Videos (Link...

...strain Watch the Video! CRISPR Library Amplification Amplify CRISPR pooled-plasmid libraries Diagnostic ...complicated procedures. Plasmid Cloning Protocols for constructing and analyzing your plasmids. Virus Protocols... that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations...Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term use Watch the Video! DNA Purification...spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes Agarose Gel Electrophoresis...Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Watch the Video! Polymerase Chain Reaction... the Video! Sequence Analysis Verify important plasmid features using sequence analysis RNA Extraction...

...Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries for CRISPR screening...

...Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries for CRISPR screening...