...stable cell lines expressing a gene of interest from an integrated lentiviral vector....stable cell lines expressing a gene of interest from an integrated lentiviral vector. Unlike the short-term...using lentiviral vectors enables long-term protein expression studies. Moreover, repeating experiments ... the most desirable level of expression. Over time, transgene expression in a polyclonal population may...and is described in Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem...Lines with Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral...short-term protein expression observed using transient transfection approaches, generating cell lines using...

...could lead to reduced transgene expression over time, as the lower expressing clones take over the polyclonal...and is described in Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem...by Limiting Dilution You may also like... Viral Vector Guides Virus Blog Posts Addgene Protocols Viral...that are more likely to retain stable transgene expression. Other methods of generating a monoclonal cell...population be monitored regularly for transgene expression. This protocol has been optimized for adherent...sufficiently expanded, screen the lines for transgene expression and/or other phenotypes. For example, perform...for lines with the highest or lowest transgene expression ( Figure 2 ). Sample Data Figure 1: Generation...