...Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing non-hairpin insert. ...: RNAi animation Whitehead siRNA Selection Program: http://sirna.wi.mit.edu/ Back to Top I. Appendix I.... pLKO.1 – scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid...Below are suggestions for target selection. Use an siRNA selection tool to determine a set of top-scoring...Whitehead Institute for Biomedical Research hosts an siRNA Selection Program that can be accessed after a free... free registration. Guidelines for designing siRNAs with effective gene silencing: Starting at 25nt downstream... parallel. This plate will serve as a positive control for the puromycin selection. Days 4+: g. Change...