...individual A549 cells stably expressing Cas9. A549 cells were transduced with lentiCas9-Blast 1 and then selected...Figure 2: Cas9 expression in monoclonal cell lines generated from A549 cells transduced with lentiCas9-Blast... not express Cas9 and are included as a control (WT). Vinculin is a loading control. 1 lentiCas9-Blast...could lead to reduced transgene expression over time, as the lower expressing clones take over the polyclonal...by Limiting Dilution You may also like... Viral Vector Guides Virus Blog Posts Addgene Protocols Viral...that are more likely to retain stable transgene expression. Other methods of generating a monoclonal cell...population be monitored regularly for transgene expression. This protocol has been optimized for adherent...

...individual A549 cells stably expressing Cas9. A549 cells were transduced with lentiCas9-Blast and then selected...stable cell lines expressing a gene of interest from an integrated lentiviral vector. Protocols...stable cell lines expressing a gene of interest from an integrated lentiviral vector. Unlike the short-term...using lentiviral vectors enables long-term protein expression studies. Moreover, repeating experiments ... the most desirable level of expression. Over time, transgene expression in a polyclonal population may...Lines with Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral...short-term protein expression observed using transient transfection approaches, generating cell lines using...

...pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed for mCherry expression using fluorescence...Assay for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral...marker. Determining the titer of your lentiviral vector allows you to control the multiplicity of infection...protocol was developed using low titer lentiviral vectors (10 5 TU/mL). If you anticipate that your viral...

...Lentivirus Production You may also like... Viral Vector Guides Mol Bio Protocols Introduction This protocol...be used to produce lentivirus from a lentiviral vector transfected into 293T cells using a polyethylenimine...Figure 1: 293T cells were transfected with the GFP-expression plasmid pRosetta using μg total DNA to μg PEI...per 10 cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would...