... additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell... Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature... events and the site(s) of proviral integration across cells. Selective pressure on this heterogeneous...been sufficiently selected with the appropriate antibiotic so that every cell in the culture has, in theory...maintain cells in selective medium at a reduced antibiotic concentration (e.g., half the concentration that...Sample Calculation First, use a hemocytometer to quantitate the homogenized cell solution. Homogenized cell...