Protocol - pLKO.1 – TRC Cloning Vector
Type
Protocol
...antisense—TTTTTG 3’ Reverse oligo:
5’ AATTCAAAAA—21bp sense—CTCGAG—21bp antisense 3’ For example...GTATAGCTTAACGTAGGCATT TTTTTG 3’ Reverse oligo:
5’ AATTCAAAAA AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT...