...website about star activity . If you are digesting a large number of plasmids with the same enzyme(s) (for... However, keep in mind that restriction enzyme activity is determined under ideal conditions with very...their recognition sites. This is due to "Star Activity" and can happen for a variety of reasons, including...double digest (digesting with two enzymes at the same time), you will need to determine the best buffer...will not be gel purifying it, you may need to inactivate the enzyme(s) following the digestion reaction...

...exonuclease function of the enzyme and shift its activity back to polymerase. In our case, dGTP will be ...protocol), causing the enzyme to perform exonuclease activity until the first "G" in the sequence. Because the...proceeding, as they will interfere with the exonuclease activity of T4 Pol in the following step. This may be accomplished...have different leader sequences, but operate on the same principle (homologous to the first G on 3'-5' strand... incubate at room temperature for 30 minutes. Inactivate T4 Pol by heating to 75° for 20 minutes. Volume...

... the appropriate antibiotics, and a section for active pouring. Prepare your antibiotics. Prior to adding...growth. See our sample data section below for positive and negative test results. Sample Data In all cases...Transfer the sterile water (in our case 220 mL) to the same bottle and swirl to form a medium/agar colloid. ...will darken during the autoclave process if your sample has spent at least 10 min at 121 ℃. Use lab tape...the autoclave and run on a setting that gets the sample to at least 121 ℃ under 20 psi for at least 30 ... any weird and wonderful organisms. While your samples are sterilizing in the autoclave, you should prepare...

...use of high voltages, please use caution when activating pulse and follow all specifications described...gel extraction of either the original sample or the amplified sample, followed by reamplification of the...tens to millions of different plasmids in a single sample. They are often used for screening, barcoding, ...appear to contain sufficient DNA for use, pool samples, and continue with analysis. “Sufficient” DNA will...

...Time (min) Ramp Rate (°C/sec) # Cycles Enzyme Activation 95 10 2 1 Denaturation 94 0.5 2 40 Annealing/...concentration of each sample on a spectrophotometer. Prepare 25 ng/µL stocks of each sample. Samples can be used...tube as shown below. For 8 samples prepare enough master mix for 9 samples. Component Volume 9X Volume...constant across samples. In the RRE example below, 2-fold serial dilutions of a sample were loaded in ...ddPCR Lentivirus sample data, RPP30 positive (green) and negative (black) droplets Sample ID Plasmid ID ... higher dilutions depending on their particular sample. This protocol was modified from the publication...Starting Thaw the master mix, primers/probe mixes and samples on ice before use. Wipe down all pipettes and surfaces...

...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...) (Link opens in a new window) (3,180 cm 2 - the same surface area as 21 x T-175 flasks). Cell stacks ... 2 Cellstack 2, Corning 3269, 1272 cm 2 Heat-inactivated FBS (HI-FBS) Pro-Tip Different brands and lots...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of glutaGRO 11 mL of 200 mM L-alanyl-L-glutamine...0.08 μg/bp Therefore, for each plasmid we need: Sample Calculations RepCap: 0.08 μg/bp × 7,265 bp = 582.1...overnight at 4 °C if needed. Transfer the entire sample to 3 x 500 mL conical bottles and centrifuge at...round of sonication to avoid overheating of the sample. Mix well between rounds of sonication. Sonicate...

...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G...bottle of DMEM high glucose, add 55 mL of heat inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C....protocol outlines the seeding of the cells at the same time as the virus-mediated transduction. For some... titer, take the average of multiple dilutions. Sample Data Figure 1: A549 cells were transduced with ...

...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5mL of 100X glutaGRO. Store at 4 °C. ...complete + 10 µg/mL polybrene. Note, this is just a sample of possible dilutions. You may want to try higher...monoclonal lines from the early polyclonal populations. Sample Data Figure 1: Generation of monoclonal cell lines...

...FBS can be purchased already heat-inactivated or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10mg/mL), Millipore TR-1003-G ...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C.... titer, take the average of multiple dilutions. Sample Data Figure 1: 293T cells were transduced with ...

...FBS can be purchased already head inactivated or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Low serum medium such as Opti-MEM or Opti-Pro...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C....24–48 h before checking for protein expression. Sample Data Legend: Lenti-X 293T cells were transfected...

...FBS can be purchased already heat inactivated or it can be inactivated in the lab by heating to 56°C for...chloroquine Polyethylenimine, linear MW 25,000 Da Heat-inactivated FBS 1x PBS pH 7.4 without calcium or magnesium...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of glutaGRO. Store at 4 °C. Pro-Tip... °C as soon as possible to avoid loss of titer. Sample Data Figure 1: 293T cells were transfected with...

...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS 1X PBS pH 7.4 without calcium or magnesium...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C....performed at Addgene on the A549 cells described in our sample data , each 96-well plate gave rise to 15–20 monoclonal...solution will be used to seed the 96-well plate. Sample Calculation First, use a hemocytometer to quantitate...plate for individual cells, which should be on the same focal plane. Scan the entire plate and make note...highest or lowest transgene expression ( Figure 2 ). Sample Data Figure 1: Generation of monoclonal cell lines...

...two different shRNAs that target the same gene can produce the same phenotype will alleviate concerns about...plasmid in mammalian cells. sin 3’LTR 3’ Self-inactivating long terminal repeat. f1 ori f1 bacterial origin...boiling water. If using a PCR machine, incubate the sample at 70°C for 10 minutes then slowly cool to room...Biotechnol . 1997. 15(9):871-5. PubMed . Self-inactivating lentivirus vector for safe and efficient in ...

...must selectively block and unblock repeatedly the reactive groups on a nucleotide when adding a nucleotide...primer is used to amplify a eukaryotic genomic DNA sample. However, a primer should not be too long (> 30...