...rapidly thaw the lentiviral aliquot on ice prior to use. Prepare dilutions of the lentivirus into DMEM complete...of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed for mCherry expression using ...Protocol that can be used to titer lentiviral preparations carrying a fluorescence marker. Protocols...Introduction This protocol can be used to titer lentiviral preparations that carry a fluorescence marker...marker. Determining the titer of your lentiviral vector allows you to control the multiplicity of infection... cell lines. It is not recommended to subject lentiviral supernatants to multiple freeze-thaw cycles. ...polyethersulfone filter to remove cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw...