...be seen here by the amount of green fluorescent protein expression (green in the right panels) with a limited...line and can be useful for short term studies of protein function. We specifically use this protocol with...with fresh media Day 3 or more (am): Observe fluorescence, harvest cells, or perform your experiment Equipment...batch of PEI is prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the ...Incubate the cells 24–48 h before checking for protein expression. Sample Data Legend: Lenti-X 293T cells...

...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...Purification Purify recombinant antibodies using Protein A or Protein G columns Watch the Video! Coomassie Purity...Watch the Video! AAV Titration by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer...Video! Western Blot Separate and detect specific proteins Watch the Video! Immunocytochemistry Use antibodies...