...with a primary antibody against a target protein and a fluorescent secondary antibody....with a primary antibody against a target protein and a fluorescent secondary antibody. This protocol outlines... expresses the protein, such as cells transfected with a plasmid to express the protein of interest, and...for fixing and labeling HeLa cells for a target protein using the formaldehyde fixation method. The protocol...need to be optimized for different cells, target proteins, etc. Sharing speeds science. We believe that ... tips and pipettes Rocking platform Tweezers Fluorescent microscope 0.5–10 µL single channel pipette 2...Paraformaldehyde fixation works well for many target proteins, it may not be the best fixation method for all...

...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...Purification Purify recombinant antibodies using Protein A or Protein G columns Watch the Video! Coomassie Purity...Video! Western Blot Separate and detect specific proteins Watch the Video! Immunocytochemistry Use antibodies...protocols that you can use for a wide range of applications, with videos for select protocols in the right-hand...preparations. Antibodies Protocols for common antibody applications. Intro to the Lab Bench Name Description (Link...