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Protocol - How to Perform a Diagnostic Digest

Type

Protocol

...Introduction Restriction enzymes are naturally occurring bacterial endonucleases that recognize a large...
Protocol - How to Inoculate a Bacterial Culture

Type

Protocol

...Glycerol Stocks for Long-term Storage of Plasmids Recovering Plasmid DNA from Bacterial Culture Background...
Protocol - Bacterial Transformation

Type

Protocol

...because bacteria are used as the means for both storing and replicating plasmids. Because of this, nearly...
Protocol - How to Ligate Plasmid DNA

Type

Protocol

...and prevents the vector from ligating to itself during the ligation process. If the sticky ends on either...
Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

Type

Protocol

...and recipient plasmids. Because you lose some DNA during the gel purification step, it is important to digest...
Virus Protocol - Generating Stable Cell Lines

Type

Protocol

... cells) into each well of the 6-well dish. This brings the total volume in each well up to 1.5 mL. Since...
Plasmid Cloning by PCR (with Protocols)

Type

Protocol

...and recipient plasmid. Because you lose some DNA during the gel purification step, it is important to digest...
Isolating a Monoclonal Cell Population by Limiting Dilution

Type

Protocol

...solution to 990 µL complete medium. Instead of transferring 0.125 µL, now transfer 100 times that volume...

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