...solution, add 2-2.5 volumes 95% or 100% ethanol and 1/10 volume of 3 M Na-acetate (pH 4.8). Invert the microfuge...Bacterial Culture You may also like... Bacterial Transformation Agarose Gel Electrophoresis Agarose Gel DNA...much as several miligrams and at concentrations ranging from 150 ng/μL to several μg/μL. The protocol below...optional) Reagents Overnight culture of bacteria transformed with your plasmid Resuspension buffer Denaturing... a pancreatic ribonuclease that digests single-stranded RNA. Optional: Perform phenol-chloroform extraction...