...of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the aqueous DNA sample. Pro-Tip...colonies and to generate liquid bacterial cultures , please see those pages. Several companies, such as (Link...remaining contaminant proteins and RNase A from the DNA sample. When phenol is mixed with the aqueous solution...

...before you place the sample at -80°C. Frozen tubes are hard to write on and samples stored for long periods...streaking onto LB agar will prevent it from thawing completely and will improve the shelf life. It is very important...

...bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow). It is, however...over-agar for selection of transformed E. coli . Sample Data Selection of E.coli on LB-agar using different...of Carbenicillin stock used (150 µL per plate). Please note we have found that there is generally a broad...

... to the BSL-1 requirements. Conclusion Although simple, following appropriate PPE protocol goes a long...reuse or repurpose this SOP in another location, please note that you do so at your own risk; you should...

...you'll find these protocols useful in your own work. Please feel free to email us at [email protected] with ...centrifuge to separate different components in a liquid sample Using a Light Microscope Learn about the parts ...

...you can weigh out the reagents in batches. For example, if you need 300 g of sucrose, you can weigh out...

...water baths with instructions on the bottle, for example, number of drops per liter. Place a thermometer...

...liquid culture. Protocol Prepare liquid LB. For example, to make 400 mL of LB, weigh out the following ...

...difficulty finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut your insert...