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Addgene
Showing: 41 - 48 of 48 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the aqueous DNA sample. Pro-Tip...remaining contaminant proteins and RNase A from the DNA sample. When phenol is mixed with the aqueous solution...

  2. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow). It is, however...over-agar for selection of transformed E. coli . Sample Data Selection of E.coli on LB-agar using different...

  3. Pipetting Protocol

    Type
    Protocol
    ...different samples without cross contamination as long as the tip is changed between samples. Tips can ...

  6. Weighing Reagents Protocol

    Type
    Protocol
    ...you can weigh out the reagents in batches. For example, if you need 300 g of sucrose, you can weigh out...

  7. Water Bath Protocol

    Type
    Protocol
    ...water baths with instructions on the bottle, for example, number of drops per liter. Place a thermometer...
Showing: 41 - 48 of 48 results