... blue mix into a cell counting chamber. Load the cell counting chamber on an automated cell counter and...conical tubes Microcentrifuge tubes Cell counting chamber slide 30 mL luer-lock syringe, BD BD302832 0.2 ...

...uses an electrical field to move the negatively charged DNA through an agarose gel matrix toward a positive...some to the buffer as well. EtBr is positively charged and will run the opposite direction from the DNA...negative, red is positive. The DNA is negatively charged and will run towards the positive electrode. Always...

...Quality Control (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but...can be used but this dramatically increases the chances of individual plasmids being lost from the pool...taken to perform adequate NGS based QC to ensure no change in representation compared to the pre-amplified...

...Iodixanol Gradient Ultracentrifugation Protocol Mulitchannel Pipetting Technique Learn how to pipette multiple...multiple samples at once using a multichannel pipette and avoid inconsistent pipetting with our simple ...

...directly interchangeable. If you need to convert between the two, use a conversion calculator or chart like...

...two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations). Generate...or follow manufacturer's instructions. You can purchase master mix or make your own. Transform bacteria...

...Design a Primer You may also like... Polymerase Chain Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis...

... culture for DNA purification will minimize the chance of having a mixture of plasmids in your purified...

... a short stretch of DNA to a plasmid, such as: Changing a Multiple Cloning Site (MCS) Adding short tags...

...primers that can be requested for no additional charge. What primers were used by Addgene during quality...

... restriction digest. Equipment Electrophoresis chamber Pipetman Pipet tips Reagents Liquid DNA aliquot...

...After incubation, check for growth, which is characterized by a cloudy haze in the media (see right). Notes...

...concentrations of insert, thereby increasing your chances of success. Set up a vector only control with water...

...restriction enzymes Watch the Video! Polymerase Chain Reaction (PCR) Basic PCR protocol with tips and ...

...disposal, commonly done with an autoclave. Only mechanical pipetting should be done in the laboratory. Don...

...the rest on a second plate. This gives the best chance of getting single colonies, while allowing you ...

...plasmid or simple template, there is very little chance for mis-priming, so you can use a pretty wide range...