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Showing: 21 - 35 of 35 results

  1. General Transfection

    Type
    Protocol
    ...transfect mammalian cells with your plasmid of interest....Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short...

  2. Gibson Assembly Protocol

    Type
    Protocol
    ...extensively. Why Gibson Cloning? No need for specific restriction sites. Join almost any two fragments regardless...and screen for the correct plasmid product by restriction digest . Sequence the important regions of your...

  3. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background... DNA: Contains the portion of DNA that we are interested in amplifying for analysis and manipulation. ...

  4. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...generate stable cell lines expressing a gene of interest from an integrated lentiviral vector....generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. Unlike the...

  5. Pouring LB Agar Plates

    Type
    Protocol
    ...your own LB/agar plates with your antibiotic of interest. Last Upload: September 14, 2016 Protocol Video...selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow...

  6. DNA Quantification

    Type
    Protocol
    ...Recovering Plasmid DNA from Bacterial Culture Restriction Digest of Plasmid DNA Background Information ...

  8. Water Bath Protocol

    Type
    Protocol
    ...temperature, catalyze chemical reactions such as restriction digests needed in molecular biology, and thaw...

  9. Fluorescence Titering Assay

    Type
    Protocol
    ...with multiple integration events leading to underestimation of the true titer. Calculate the transduction...

  15. AAV Production in HEK293 Cells

    Type
    Protocol
    ...pRC (Rep-Cap), plasmid expressing your gene of interest Triton X-100 Benzonase/DNAse I (Millipore 71205...
Showing: 21 - 35 of 35 results