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  1. Gibson Assembly Protocol

    Type
    Protocol
    ...other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed... product by restriction digest . Sequence the important regions of your final plasmid, particularly the...

  2. Immunocytochemistry

    Type
    Protocol
    ...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: January...

  3. Pipetting Protocol

    Type
    Protocol
    ...Conclusion Pipetting accurately and precisely is important since even small changes in the volume could affect...Beyond knowing the proper way to pipette, it’s important for scientists to calibrate their pipettes to ...

  4. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...organelles, lipoproteins, and macromolecules. Importantly, iodixanol is inert and non-toxic to mammalian...and unlike other density gradient media, it is reportedly not necessary to remove iodixanol prior to use...

  5. Pouring LB Agar Plates

    Type
    Protocol
    ... of 10 mL, we’ll want 200 mL of media total. Importantly, we’ll actually make a bit more than 200 mL (...kill most potential contaminants, certain types of spores will stubbornly survive even under the harsh conditions...

  6. Western Blot

    Type
    Protocol
    ...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. General Guidelines ...

  7. AAV ddPCR Titration

    Type
    Protocol
    ...place the PCR plate with the foil onto the metal support block. Place the block in the plate sealer and ... reading. When the droplet reading is complete, export the data from all wells as a CSV file and use the...

  8. Kit Free RNA Extraction

    Type
    Protocol
    ...Air-dry the pellet for 5-10 minutes. Critical It is important to not let the pellet get too dry before resuspending...

  9. DNA Quantification

    Type
    Protocol
    ... different stages of molecular cloning, it is important to get a quick and accurate reading of DNA concentration...

  10. Protocol - How to Design Primers

    Type
    Protocol
    ...toward one another. The size of the primer is very important as well. Short primers are mainly used for amplifying...

  12. Protocol - How to Streak a Plate

    Type
    Protocol
    ...initials. Labeling within a laboratory setting is important for organization, and it is recommended that you...

  13. Weighing Reagents Protocol

    Type
    Protocol
    ...Conclusion Appropriately weighing reagents is important to ensure the accurate composition of your solutions...

  15. Ligation Independent Cloning

    Type
    Protocol
    ...provided by your polymerase manufacturer. It is very important to remove all free nucleotides from your PCR product...

  16. Video Library

    Type
    Protocol
    ... office, discusses her career in science, the importance of international collaboration, and offers career...

  18. Fluorescence Titering Assay

    Type
    Protocol
    ...CI) Heat-inactivated FBS Polybrene (10mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium...

  20. Lentivirus Production

    Type
    Protocol
    ...transfection efficiencies as measured by the highest proportion of GFP positive cells without limiting cell growth...
Showing: 21 - 40 of 43 results