...risk of contaminating reagents we recommend making small aliquots of master mixes, primers, and probes prior...302411100 Droplet Digital PCR System, Bio-Rad, DX200 Thermal Cycler, Bio-Rad, T100 PCR Plate Sealer, Bio-Rad...plate has been sealed, proceed to thermocycling. Thermal Cycling Run the following PCR parameters. Cycling...

...accuracy as these types of scales display more decimal places. Pro-Tip If you’re weighing out an amount...amount of reagent that will be resuspended in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may...

...time (approximately 18-30 hr). On the other hand, smaller plasmids can be present in large numbers, 50 or...may help to increase the density of the culture. Normally cultures shake at 150 - 250 rpm, increase this...

...sterile water (instead of TE buffer) to ensure optimal salt concentrations in subsequent reactions. Step...per annealing reaction. This should be done in a small volume with no additional water (<5 μl). It is advisable...

...the bacteria back into suspension, but within a smaller volume of buffer that is compatible with the next... genomic DNA to precipitate, while leaving the smaller plasmids free in solution. Pellet the proteins ...

...~220 mL) just in case we spill anything or have small errors in measurement. You should also always make...-mix slightly. Even so, you should always use thermally insulated gloves when removing anything from the...

... Short primers are mainly used for amplifying a small, simple fragment of DNA. On the other hand, a long...

...plasmid DNA prep the following day. Tips and FAQ The optimal concentration of long-term glycerol storage is ...

...toothpicks or wire loop Bunsen burner (or other small flame source) Incubator Marker Reagents LB agar ...

...higher percentage agarose gel will help resolve smaller bands from each other, and a lower percentage gel...

...-8 pieces such that all (or most) fragments are small enough to be accurately sized on a gel and also ...

...height. They often spin at similar speeds to their smaller cousins, but can hold much larger containers. Ultracentrifuges...

... hour. Using this ratio, you can calculate the minimal amount of enzyme for your reaction. However, keep...

...for most standard cloning (where the insert is smaller than the vector) a 3 insert : 1 vector molar ratio...

...discard the tube and thaw a new working stock. The optimal mass DNA:mass PEI ratio will need to be empirically...

...the tube and vortex for 5 s to mix. Pro-Tip The optimal ratio of DNA:PEI may vary significantly and should...

...Importantly, iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other density gradient...

...higher percentage agarose gel will help resolve smaller bands from each other, and a lower percentage gel...

...be placed into a backbone vector of choice with minimal limitations. Background In its simplest form, PCR...

...mix. Centrifuge at 3100 x g for 8 min . Remove a small aliquot of your sample from the filter reservoir...