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  1. Kit Free RNA Extraction

    Type
    Protocol
    ... working with the volatile reagents in the list above. Procedure Option #1 - Solution D Protocol Before...sure to prepare solution D (see reagent section above for the recipe). If using TRIzol®, jump down to ...

  2. Lentivirus ddPCR Titration

    Type
    Protocol
    ...the titer. Calculations In the experimental setup above, the following virus dilutions were used to transduce...v = Virus volume, mL *In the experimental setup above, the cells per well is 300,000 and the virus volume...

  8. Gibson Assembly Protocol

    Type
    Protocol
    ... the right). When designing your plasmid, think about what DNA segments you will need to join to create...

  14. AAV ddPCR Titration

    Type
    Protocol
    ...dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer in a microcentrifuge...

  15. CRISPR Library Amplification

    Type
    Protocol
    ...require modifications dictated by the originating laboratory for optimal results. If you obtained the pooled...
Showing: 21 - 35 of 35 results