...promoter is included in the pSico plasmid to drive shRNA expression. Other features that can be included ...those that can are primarily third generation constructs. Lentiviral transfer plasmids are not designed... lentiviral transfer plasmids normally used for shRNA expression? Yes, it is feasible, but first the promoter...within the transfer plasmid must be modified. Most shRNA‐expressing lentiviral plasmids, such as pLKO.1, ... to drive RNA pol III-directed transcription of shRNAs. cDNA expression requires RNA pol II, and thus ...envelope, and transfer vectors, and the resulting construct is packaged into a viral particle, it is not possible...

...transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is flanked by...wildtype LTR promoter is no longer present in these constructs. Why do many γ-retroviral plasmids have similar...

...the RNA polymerase. A transcription complex is constructed from the RNA polymerase and several transcription...Polymerase III also transcribes small RNAs, such as shRNAs and gRNAs. Resources Addgene's blog , including...

...interested in. Self-replicating - Once you have constructed a plasmid, you can easily make an endless number...frequently accomplished through expression of an shRNA targeting the mRNA of the gene of interest. These...