...Most shRNA‐expressing lentiviral plasmids, such as pLKO.1, use a U6 or H1 promoter in order to drive RNA ...detailed protocol for using the popular cloning vector pLKO.1. Trono Lab Resources: Lentivectors Toolbox and ...lentiviral transfer plasmids are based on the HIV-1 virus. For safety reasons, transfer plasmids are all...selection. Desfarges S, Ciuffi A. Viruses. 2010. Jan;2(1):111-30. PubMed . Altering the tropism of lentiviral...Curr Gene Ther. 2005. 5(4): 387-398. PubMed . HIV-1 Genome Nuclear Import Is Mediated by a Central DNA...