...these principles apply to using CRISPR in other organisms. First, consider the genetic manipulation that...carry out your experiment in your particular model organism. There may not be a perfect plasmid for your specific...disrupt gene function in a particular cell type or organism without a specific preferred mutation Cas9 (or...effects Additional Resources: CRISPR protocols from Addgene depositors JOVE Video Reprint: Protocol for genomic...sequence and design your gRNA. Know your cell line/organism and genomic sequence When possible, you should...may vary depending on the specific cell line or organism, which may affect the observed efficiency of CRISPR...containing validated gRNAs are now available through Addgene. These plasmids contain gRNAs that have been used...

...transcriptional machinery from a particular organism or group of organisms. Meaning, if a plasmid in intended ...videos for specific techniques and tips! Addgene Protocols Addgene Video Library...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing...abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix. The order of these...the instructions to make every protein within an organism. Proteins are made up of amino acids. Each amino... plasmid. For commonly used primers check out Addgene's sequencing primer list. Working with Plasmids ...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured...

...types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you about AAV components,...information on optogenetics plasmids available from Addgene. The popular CRISPR/Cas9 genome editing system ...cycle. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...optimal serotype(s) for transduction of a given organ. Tissue Optimal Serotype CNS AAV1, AAV2, AAV4, AAV5...Additional Resources AAV Plasmids Available at Addgene Biosafety Guide Parts of an AAV Transfer Plasmid...

...Addgene's molecular biology reference material covering promoters, transcription and the transcription...promoter region controls when and where in the organism your gene of interest is expressed. Summary Promoters...small RNAs, such as shRNAs and gRNAs. Resources Addgene's blog , including our popular Plasmids 101 series...

...plasmids available from Addgene Find ready-to-use lentivirus available through Addgene's viral service Read...integration. Packaging plasmid(s) Envelope plasmid Addgene’s packaging and envelope plasmids are generalized...reporters. Browse lentivirus plasmids available from Addgene. 2nd Generation The graphic to the right shows ...information about cloning and changing a MCS, visit Addgene's Plasmid Reference and Protocol Guide . How do ... 3rd generation lentiviral systems provided by Addgene separate transfer, envelope, and packaging components...transfer vectors that have been deposited with Addgene are self-inactivating (SIN) vectors. These vectors...Spring Harbor, 1997. Available online: Genetic Organization and Genetic & Structural Features . In vivo ...

...Addgene's guide to using Chemogenetics plasmids in your lab for interrogation of neuronal activity.... find chemogenetics plasmids available through Addgene, check out our Chemogenetics Plasmid Collection...that both KORD and hM3Dq can be used in the same organism to allow bidirectional control of neuronal activity...alternative DREADD ligands, please see the text. Browse Addgene's collection of DREADD plasmids ! PSAMs Unlike DREADDs...Neuronal activation Magnus et al., 2011 Browse Addgene's collection of PSAM plasmids ! LMOs Luminopsins...Neuronal inhibition Ikefuama et al., 2025 Browse Addgene's collection of LMO plasmids ! Planning Your Chemogenetics...

...Addgene's guide to using Optogenetics plasmids in your lab, including explanations of microbial opsin...To find optogenetics plasmids available through Addgene, check out our Optogenetics Plasmid Collection ...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...previous tools through directed mutagenesis - use Addgene's Optical Switch Plasmid Table to search for plasmids...

...window) . Addgene Website & Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101...cell lines Viral service at Addgene Viral plasmids and resources at Addgene Blog CRISPR topic page How ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...You Use a CRISPR Library? CRISPR libraries from Addgene are available in two formats: as liquid DNA, or...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...located. The majority of the CRISPR plasmids in Addgene’s collection are from S. pyogenes unless otherwise...

...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...intensive. Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...