...light-emitting luciferase and a light-sensing optogenetic element. When the luciferase substrate (luciferin...control expression of a chemogenetic reporter ensures that the reporter remains silent until a cell or tissue-specific...luciferin) is added, the luciferase enzyme generates light that activates the opsin. The opsin may excite...Coupling brighter bioluminescent modules, such as luciferase-fluorescent protein fusions, to more-sensitive...increased efficiencies. Depending on the type of luciferase used, the luciferin substrate may be the native...Descriptions of LMOs and their activity in neurons LMO Luciferase Opsin Ligand Effect Outcome (in neurons) Reference...

...elements. These plasmids contain a reporter gene (for example, luciferase or GFP) that offers a read-out ...information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for studying the function of genetic...of interest could be inserted upstream of the luciferase gene to determine the level of transcription ...