...destination vector (in this case the destination vector would be the lentiviral transfer vector). The destination...What is the difference between a lentivirus and a retrovirus? Lentiviruses are a subtype of retrovirus. From...difference between lentiviruses and standard retroviruses (γ-retroviruses) is that lentiviruses are capable ...that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and ...in order to produce lentivirus? 293T cells are usually used to produce lentivirus. What dictates lentiviral...their tropism . How can lentivirus be used to make stable cell lines? Lentiviruses can be used to make stable...does lentivirus integrate? Genome-wide studies of viral integration have shown that lentiviruses most ...

...preferred over lentiviruses because they remain primarily episomal, while lentiviruses integrate into ...References Vector Biolabs: Introduction to AAV University of South Carolina's Viral Vector Core AAV Overview...gene delivery vectors: molecular engineering and evolution of adeno-associated viral vectors for enhanced...3(4):281-304. PubMed . Vector genome integration: Adeno-associated virus vector integration. Deyle DR,...-99. PubMed . Adeno-associated virus serotypes: vector toolkit for human gene therapy. Wu Z, Asokan A,...Epub 2006 Jul 7. PubMed . Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1...;10(2):302-17. PubMed . From virus evolution to vector revolution: use of naturally occurring serotypes...

...Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune system, antibodies...restricted to certain gene sets. Read More Viral Vectors Find educational guides to learn more about the...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus...

... in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP...lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression ...Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for details...oligos and clone these oligos into your desired vector. In many cases, targeting oligos are synthesized... exact cloning strategy will depend on the gRNA vector you have chosen, so it is best to review the protocol...

...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual... included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...