...Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top Type IIS Assembly (Golden Gate & MoClo... next cloning project. Golden Gate Cloning (Image from Plasmid 101: Golden Gate Cloning ) Back to Top ...on TOPO cloning. Back to Top Gibson Assembly (Isothermal Assembly Reaction) Isothermal cloning, more commonly... MoClo) Type IIS systems, such as Golden Gate and Modular Cloning (MoClo) , take advantage of the unique... to assemble before you get started. Check out Addgene's website for easy to use MoClo and Golden Gate...Gibson assembly DNA fragments with 20-40 base pair homology at their ends can be easily ligated together... commonly known as Gibson assembly ( protocol ), takes advantage of the properties of 3 common molecular...