...Top Type IIS Assembly (Golden Gate & MoClo) Type IIS systems, such as Golden Gate and Modular Cloning (...to use MoClo and Golden Gate cloning kits for your next cloning project. Golden Gate Cloning (Image from...from Plasmid 101: Golden Gate Cloning ) Back to Top Ligation Independent Cloning Ligation Independent ...Back to Top Gateway® Recombination Cloning Gateway cloning (Image from Plasmid 101: Gateway Cloning ) Gateway... Gateway® cloning is a recombination based cloning method. The benefit of Gateway® is that moving a piece...cassettes). Although gateway cloning is a simple and efficient cloning method, gateway vectors and recombination...Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO Gibson Type IIS Ligation Independent Cloning...