...exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top Type IIS Assembly (Golden...post on TOPO cloning. Back to Top Gibson Assembly (Isothermal Assembly Reaction) Isothermal cloning, more...more commonly known as Gibson assembly ( protocol ), takes advantage of the properties of 3 common molecular... DNA ligase fuses DNA strands together. In Gibson assembly DNA fragments with 20-40 base pair homology...DNA. A major benefit of Gibson cloning is that it allows for the simple assembly of multiple fragments ...cloned into. A drawback of this system is that Gibson assembly works best when combining DNA fragments over...Engineering Guide Restriction Enzyme Gateway TOPO Gibson Type IIS Ligation Independent Cloning Oligo Stitching...