...another marker, such as GFP. A researcher can use FACS to sort cells expressing GFP and later expand these...produce lentivirus? 293T cells are usually used to produce lentivirus. What dictates lentiviral host cell... Guides Lentiviral Guide Lentiviral Guide...FAQ References Glossary Lentiviral Components To increase the safety of lentivirus, the components necessary... below. Third Generation Lentiviral Plasmids 2nd vs 3rd Generation Lentiviral Systems Feature 2nd Generation...generation lentiviral systems? For a full description of 2nd and 3rd generation lentiviruses, please review...What is the difference between a lentivirus and a retrovirus? Lentiviruses are a subtype of retrovirus. From...

...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable,...high efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector...

...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic...these plasmids to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that...viral service offers select ready-made AAV and lentiviral particles. Visit our viral service page to learn...

...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...