...Photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains...release proteins from cellular membranes using light. light-induced protein dissociation using protein A fragments...photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins offer exquisite ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...Inhibits protein function by reversibly sequestering targets into large, optically assembled protein architectures...transcription using engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage...pair to the target protein, and the other to the membrane where the target protein is to be sequestered...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...; or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...

...transmembrane proteins that bind a specific ligand. Ligand binding then activates G-proteins to modulate...signaling protein that the receptors couple to: Gq-DREADDs signal through the Gαq/11 G-protein and activate...bioluminescent modules, such as luciferase-fluorescent protein fusions, to more-sensitive opsins has led...The first chemogenetic receptors were based on G-protein coupled receptors, or GPCRs. The largest class ...into chemogenetic tools. Need a refresher on G-protein coupled receptors? Read the GPCRs: How Do They ...DREADDs), like RASSLs, are based on engineered G-protein coupled receptors, but unlike RASSLs, show insensitivity...employed. Gi-DREADDs signal through the Gαi/o G-protein and inhibit neuronal signaling by inhibiting adenylate...