...Overlay Select Fluorescent Proteins 101 August 2017 | First Edition About Fluorescent Proteins 101 August...for choosing a fluorescent protein; information on FRET, biosensors, and monitoring protein localization...localization; an introduction to non-protein fluorophores; and much more! Close About Overlay Select Viral Vectors...contains an introduction to each vector and its applications, tips for using vectors in your experiments,...your transferable skills, and tips on the job application process. Close About Overlay Select CRISPR 101...introductions and helpful tips for antibody-based applications, from flow to IHC! Close About Overlay 0 of ...

...provides the instructions to make every protein within an organism. Proteins are made up of amino acids. Each...control of a non-bacterial promoter) or a fluorescent protein (that can be used to select or sort the cells...recognizes the different fluorescent labels. The machine detects which fluorescently labeled nucleotide is...discoveries lead to the conclusions that genes encode proteins and reside on chromosomes, which are composed ...in the central dogma of molecular biology, that proteins are translated from RNA, which is transcribed ...Although plasmids were originally used to understand protein coding gene function, they are now used for a variety...enhancer sequence which increases the amount of protein or RNA produced. Expression vectors can drive expression...

...Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins 101 Science Career Guide Videos Watch our...techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology ...written and video protocols for a wide range of applications in molecular biology, plasmid cloning, and virus...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...specificity and applicability of base editing through protein engineering and protein delivery. Nature...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...; or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...

...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...primarily show a protein’s presence or absence. Figure 3: A western blot detecting proteins using the indirect...

...release proteins from cellular membranes using light. light-induced protein dissociation using protein A fragments...Photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains...photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins offer exquisite ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...Inhibits protein function by reversibly sequestering targets into large, optically assembled protein architectures...transcription using engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage...pair to the target protein, and the other to the membrane where the target protein is to be sequestered...

...transmembrane proteins that bind a specific ligand. Ligand binding then activates G-proteins to modulate...signaling protein that the receptors couple to: Gq-DREADDs signal through the Gαq/11 G-protein and activate...bioluminescent modules, such as luciferase-fluorescent protein fusions, to more-sensitive opsins has led...The first chemogenetic receptors were based on G-protein coupled receptors, or GPCRs. The largest class ...into chemogenetic tools. Need a refresher on G-protein coupled receptors? Read the GPCRs: How Do They ...DREADDs), like RASSLs, are based on engineered G-protein coupled receptors, but unlike RASSLs, show insensitivity...employed. Gi-DREADDs signal through the Gαi/o G-protein and inhibit neuronal signaling by inhibiting adenylate...