...pre-processing form of crRNA is expressed and a tracrRNA (the helper RNA that facilitate the processing... then when expressed in cell and with Cas9 and tracrRNA, it will be processed (i.e., cleaved) into a shorter...form of crRNA is fused chimerically to a mature tracrRNA), the spacer used is only 20bp. Please refer to...

... : Generates crRNA for use in combination with tracrRNA. 51434 pHD-DsRed : vector for generating dsDNA...

...repeat motifs (squares), and also carrying the tracrRNA sequence (not shown). (B) Relative GFP and RFP...

...trans-activating crRNA (tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse...together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual...individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out the DNA sequence complementary...cleaves both strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break...

...CRISPR RNA (crRNA) and the trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also... cassettes to express the CRISPR RNA array and tracrRNA separately (Cong et al ., Science 2013). Additionally...or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes...

...RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) ; PDF 248.6...

...synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both targeting specificity and scaffolding...