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Showing: 1 - 5 of 5 results

  1. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Hexahistidine tag with TEV cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV cleavage, AmpR 26094... with TEV cleavage, KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR ...thioredoxin with TEV cleavage, KanR 26107 pNIC-ZB GU452710 Hexahistidine tag , Z-basic, TEV cleavage, AmpR 26108...with Thrombin cleavage, KanR 26096 pET28-MHL EF456735 Hexahistidine tag with TEV cleavage, KanR 26098 pCW-LIC... pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector...proteins with N-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR 26100 pFHMSP-LIC-C...proteins with C-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR Related ...

  2. Bacterial Expression Systems

    Type
    Collection
    ...lactose/IPTG inducible) N terminal GST tag with TEV cleavage site to remove tag Nicola Burgess Brown pGEX...expression vector with N-terminal GST tag and TEV cleavage site. Includes sites for LIC cloning, and a ..., Strep, mOCR, MBP, with and without SUMO or TEV cleavage sites to remove tags Scott Gradia Arabinose ...Strep, mOCR, MBP, GFP, with and without SUMO or TEV cleavage sites to remove tags. Scott Gradia Lactose/IPTG...26106 T7 LacO (lactose/IPTG inducible) N terminal, TEV cleavable 6x-His tag Ophear Gileadi pET-28-a based...based expression vector containing N-terminal, TEV cleavable, 6x-His and thioredoxin tags. Other empty vectors...26092 T7-lacO (lactose/IPTG inducible) C-terminal TEV cleavable 6x His Cheryl Arrowsmith Empty lactose/...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Gitler 29629 GST-TEV-FUS FUS TEV, GST T7 ALS Aaron Gitler 29630 GST-TEV-FUS-267-526aa FUS TEV, GST T7 ALS ...29631 GST-TEV-FUS-1-373aa FUS TEV, GST T7 ALS Aaron Gitler 29632 GST-TEV-FUS-1-413aa FUS TEV, GST T7 ALS...pGST2-GST-TEV-OPTN S177D S473D OPTN GST-TEV ALS Sascha Martens 187830 pFastBac_Dual_GST-TEV-EGFP-TBK1 ...pET-Duet1-His-TEV-mCherry-OPTN WT OPTN His, TEV, mCherry ALS Sascha Martens 190192 pET-Duet1-6xHis-TEV-OPTN OPTN...-GST-TEV-mCherry-TBK1 TBK1 mCherry, GST, Tev P10 ALS Sascha Martens 198037 pFastBac_Dual_GST-TEV-EGFP-...666) TBK1 GFP, GST, Tev P10 ALS Sascha Martens 198428 pGST2-GST-TEV-OPTN OPTN TEV, GST tac ALS Sascha ...PRKCG PRKCG His, TEV T7 Spinocerebellar ataxia 18 Nicola Burgess-Brown 39042 GAK GAK TEV, His T7 Parkinson's...

  5. CRISPR Guide

    Type
    Collection
    ...target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage. Generating...domains, RuvC and HNH, can be altered to reduce cleavage events to a single cut (nick) or no cuts. Cas9...nuclease domains, D10A and H840A, resulting in no cleavage of target DNA. Cas9n increases target specificity... target DNA based on the gRNA sequence without cleavage. This means proteins with other functions can ...incorporating mutations that prevent further Cas9 cleavage will improve the accuracy and efficiency of the...with larger edits using HDR. Cas12a-mediated DNA cleavage creates DSBs with a short 3′ overhang, allowing...non-specifically after the initial crRNA-guided cleavage, which slows bacterial cell growth and may further...
Showing: 1 - 5 of 5 results