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Showing: 1 - 13 of 13 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ...Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources... LTR promoters ; see article for lentiviral vectors for tet-inducible gene or miRNA expression rtTA On...Resources: Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections...puromycin selection TetR On Wiederschain 41393 pCW57.1 Lentiviral vector for inducible expression for Gateway...Off PI 21916 Tet-pLKO-neo 3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin... rtTA On Root 11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene or shRNA; see...None On Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression of a miR-shRNA; selection...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... vector - Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA...Neomycin (G418) Mammalian Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pLenti...hygro - Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral... Lentiviral Gateway destination vector for gene expression (tet inducible) Zeocin...Zeo DEST - Tet-inducible lentiviral Gateway destination vector for gene expression...expression pWPXL - Lentiviral expression pBI-MCS-EGFP - Tet-inducible Transient ... bacterial expression vector (see tagged expression vectors from the SGC) pPro18...

  3. Lentivirus Plasmids

    Type
    Collection
    ...versions of this plasmid. Root 44012 pInducer20 3rd Tet-inducible lentivirus for ORF expression, multicistronic...Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA plasmid (pGIPz based) for inducible knockdown,...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material... selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection...

  4. Cre-lox system

    Type
    Collection
    ... pBS537 tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre...D_creERt2 Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian...Nxph4-2A-CreERT2 Targeting Vector Cre-ERT2 - Tamoxifen inducible; Targeting vector Mouse Nxph4 Mammalian Zeng...Tamoxifen inducible; Targeting vector for Pax7 Tanaka 111187 pBADZ-HisCre Cre; arabinose inducible. PBAD ...components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre...promoterless vector with several available restriction sites none Mammalian Sauer 11957 pBS595 tet-hCMV-EGFPcre...tet-hCMV-EGFPcre Cre-EGFP fusion Tet inducible Mammalian Sauer 11958 pBS532 promoterless GFPcre promoterless CRE-GFP...

  5. Mammalian RNAi Tools

    Type
    Collection
    ...expression Tyler Jacks Tet-Regulated Lentiviral shRNA Lentiviral vectors for Tet-regulated shRNA expression...for RNAi Constitutive Lentiviral Cre-regulated Lentiviral Tet-regulated Lentiviral Constitutive Retroviral...design and delivery. Constitutive Lentiviral shRNA Lentiviral vectors for constitutive shRNA expression...system. Didier Trono Cre-regulated Lentiviral shRNA Lentiviral vectors for conditional (Cre-lox) shRNA expression...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...those that allow for conditional (Cre-lox) or inducible (Tet) expression are available. To find plasmids...cassette is included in the vector to monitor expression; Designed for inducing RNAi in a wide range of cell...

  6. Retrovirus Plasmids

    Type
    Collection
    ...γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus... Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian...retroviral plasmid Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See...

  7. Neurodegeneration Research Collection

    Type
    Collection
    ...reporter and a PAX7 inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant...bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...

  8. Plan Your Experiment

    Type
    Collection
    ...expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 ...efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector or separate...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable,...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for details...oligos and clone these oligos into your desired vector. In many cases, targeting oligos are synthesized...

  9. Qi Lab CRISPR Page

    Type
    Collection
    .../ARS vector (Leu2) that contains dCas9 fused to N 51025 pSLQ1661-sgMUC4-E3(F+E) Lentiviral vector that...bacteria ) contains an anhydrotetracycline (aTc)-inducible dcas9 gene on a p15A vector with chloramphenicol...CRISPR imaging 51024 pSLQ1651-sgTelomere(F+E) Lentiviral vector with sgRNA targeting human telomeres 46913...endogenous TEF1 promoter 46923 pSNR52-sgTET Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled...constitutive sgRNA expression cassette on a ColE1 vector with ampicillin resistance, wherein the N20 can...constructs 46910 pHR-SFFV-dCas9-BFP Human expression vector containing SFFV promoter, dCas9 that is fused to... 46911 pHR-SFFV-dCas9-BFP-KRAB Human expression vector containing SFFV promoter, dCas9 that is fused to...

  10. Plasmid Collections

    Type
    Collection
    ...Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits are groups...Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus...

  11. CRISPR Guide

    Type
    Collection
    ...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147. PMID...8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9...positively-charged grooves on Cas9. gRNA binding induces a conformational change in Cas9, shifting it into...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...

  12. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural... Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells...-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP. Nat Commun. 2018 Apr...exocytosis and spine enlargement during chemically induced long-term potentiation. J Neurosci. 2006 Feb 15... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal...ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature. 2015 Aug 20;524(7565):309-14...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off Full...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...Romet-Lemonne 92200 pINDUCER20-delta-Syn SNCA Myc TRE2 Parkinson's Huda Zoghbi 92201 pINDUCER20-Tau MAPT Myc ...Ataxia Richard Youle 176485 Piggybac-TetO-hNurr1-blast NR4A2 TetO Parkinson's Marius Wernig 176852 UBQLN2...Ronald Hart 190811 pTet-O-APOE-E2-T2A-Puro APOE UbC Alzheimer's Ronald Hart 190812 pTet-O-APOE-E3-T2A-Puro... CMV Ataxia telangiectasia Stephen Elledge 43918 tetO-ALN NR4A2 His, V5 TRE Parkinson's John Gearhart ...
Showing: 1 - 13 of 13 results