...Kissler 12247 pLVTHM Expresses shRNA under the H1 promoter; shRNA or a H1-shRNA cassette from another vector...causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression Tyler Jacks... both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression Tyler Jacks...browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...for more information on shRNA design and delivery. Constitutive Lentiviral shRNA Lentiviral vectors for...constitutive shRNA expression ID Plasmid Description PI 10878 pLKO.1 ‐ TRC cloning vector shRNA expression...1864 pLKO.1 ‐ scrambled shRNA Negative control vector containing scrambled shRNA David Sabatini 11795 pLL3.7...

...expression of shRNAs; addition of Cre turns on shRNA expression. See article for more cre-lox shRNA expression...the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...glycoprotein (Indiana strain). Use with packaging construct encoding Tat. Reiser 17531 pCD/NL-BH*DDD 2nd Packaging...GP. Good for work in human MSCs. Use packaging construct encoding Tat. Reiser Return to Top Popular Lentiviral...Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. See plasmid ...10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning...cloning Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker. See plasmid 14749 for Thy1.1...

...pAAV-Ptet-RFP-shR-rtTA AAV; shRNA cloning vector; for evaluation of shRNA efficacy using fluorescence;...for additional constructs TetR On Geley Transactivators (tTA or rtTA) Find a construct that expresses ...Tetracycline-inducible Empty Backbones Find a construct that will allow you to insert and induce your ... lentiviral plasmid for inducible expression of shRNA; neomycin selection; plasmid 21915 has puromycin...vector for inducible expression of transgene or shRNA; see article for detailed information about cloning...Lentiviral vector for inducible expression of a miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see...On Fraser 44012 pInducer20 Lentiviral vector for shRNA expression; see article for additional tookit plasmids...

...Cre-dependent shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre expression...switch CMV Mammalian Livet loxP (floxed) Constructs These constructs allow for Cre-regulated gene expression...Depending on the construct, Cre may activate or repress gene expression. Common construct types include:... expression promotes shRNA expression. Gene Switch: These constructs contain two genes of interest, Genes...Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs Cre Reporters & Tools Additional Resources References...recombination principles, scientists have developed constructs to activate/inactivate genes when Cre is present...

... Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression ... shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression ...Retroviral shRNA expression pSico PGK GFP - Conditional shRNA expression through... different genetic element or want to express shRNA. Choose by: Species-specific expression Epitope ...markers Viral expression and packaging Reporters, shRNA expression, transgenics and genome modification...Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pLenti CMV Neo DEST - ...expression pMKO.1 puro - Retroviral shRNA expression pLenti CMV Puro DEST - Lentiviral...

...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro ...cytoplasm Hahn 11375 MDH1-PGK-GFP_2.0 MSCV Retroviral construct for miRNA expression Chen 52114 pMSCV-IRES-mCherry...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional...

...leukoencephalopathy David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335...28195 tdp43-EGFP construct2 TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP CMV ALS...28197 tdp43-EGFP construct4 TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP CMV ALS...28199 tdp43-EGFP construct6 TARDBP GFP CMV ALS Zuoshang Xu 28200 tdp43-EGFP construct7 TARDBP GFP CMV ALS...28201 tdp43-EGFP construct8 TARDBP GFP CMV ALS Zuoshang Xu 28202 tdp43-EGFP construct9 TARDBP GFP CMV ALS...28203 tdp43-EGFP construct10 TARDBP GFP CMV ALS Zuoshang Xu 28204 tdp43-EGFP construct11 TARDBP GFP CMV ...Josef Kittler 163858 VPS35 shRNA1 VPS35 Parkinson's Lei Lu 163859 VPS35 shRNA2 VPS35 Parkinson's Lei Lu ...