... Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178 Human... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition library...human genes along with 500 non-targeting controls Puromycin Doench , Root Mouse knockout pooled libraries ... genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can...

...can use a selection marker to select transfected cells (for example, plasmid with a puromycin resistance...your HR template has a selection marker such as puromycin, you can (also) select for the positive colonies...might still be okay if you are introducing a selection marker gene. The homology arms should be no more ...colonies by puromycin selection. You could then confirm this purification by performing a genotyping assay...sequence. Because the 'NGG' of the PAM is used to select your genomic target, you need to make sure the ...protospacer followed by PAM. Click here for a target selection tool that should be helpful in minimizing or testing...clone in your target guides, test run them and then select the best guide to co-transfect in HR donor. Alternatively...

...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication... epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid contains Cas9. ID...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6...-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222 pPBT-PE2-PuroTK-pegRNA_GG...

...inducible expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection. Wiederschain 11578 pSico...shRNA empty plasmid with EGFP marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro...or miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau ...-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible...19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for ...Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain...

... by species, epitope tags/fusion protein, selectable marker, viral expression, and more.... expression Epitope tag or fusion protein Selectable markers Viral expression and packaging Reporters,... list of common tags . Back to Top Selectable Markers Regardless of your delivery method, it's ...Thus, many plasmids have markers on them so that you can find or select for only the cells that received... received the plasmid. Selectable Marker Typical Host Organism Representative... gene expression, multiple selectable markers pMKO.1 puro - Mammalian retroviral...retroviral gene expression with GFP selectable marker LZRS-Rfa or pMXs-gw - Mammalian retroviral...

...Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker or cloning method...Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu 45946 pU6-BbsI-chiRNA dU6...

...) MSCV For cloning and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...-IRES-GFP MSCV For transgene expression and GFP marker Reya 21654 pMSCV PIG (Puro IRES GFP empty plasmid...

...plasmid ID 48139), which include GFP and puromycin as selectable markers, respectively, or constructs in which... sgRNA and SpCas9, but does not contain markers for selection 1 . Other constructs may be utilized, such...Screening CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a...conditions optimized on the bulk cells (see step 6 ). Select the clones identified with the desired deletion...onto LB agar plates with the relevant antibiotic. Select multiple colonies, mini-prep each one, and subject...initial screen ensures that the correct clone was selected and reproducibility of results. To evaluate the...