Skip to main content
Addgene
Showing: 1 - 7 of 7 results

  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...mCherry Kuhn pU6-(BbsI)_CBh-Cas9-T2A-BFP 64323 Mammalian BbsI yes, cut S. pyogenes BFP Kuhn pU6-(BbsI)_CBh-Cas9...Zhang phH1-gRNA 53186 Mammalian BbsI none S. pyogenes Gersbach pmU6-gRNA 53187 Mammalian BbsI none S. pyogenes...Gersbach phU6-gRNA 53188 Mammalian BbsI none S. pyogenes Gersbach ph7SK-gRNA 53189 Mammalian BbsI none S. pyogenes...U6:1_U6:3tandemgRNAs 49411 Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pU6-BbsI-chiRNA ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...genome. gRNA Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector...pyogenes Musunuru pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI) 64217 Mammalian BbsI yes, cut S. pyogenes mCherry...

  2. FlyCRISPR

    Type
    Collection
    ...cloned by annealed oligos into the pU6-BbsI-chiRNA plasmid via the BbsI restriction sites. Design oligos... al. (2013). Plasmid is low copy number. 45946 pU6-BbsI-chiRNA : Plasmid for expression of chiRNA under...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Harrison Lab - ...template: example : Resources: Protocol for generating gRNAs 106.5 KB flyCRISPR website contains additional information...information CRISPR Optimal Target Finder Identifies gRNA target sequences from an input sequence and checks...3xP3-DsRed. (Also known as pHD-DsRed-att). 51026 U6-BbsI-crRNA : Generates crRNA for use in combination with...

  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...Mikkelsen 174038 pU6-tevopreq1-GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...nicking sgRNA BbsI No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI ...BbsI for sgRNAs; BbsI + PstI for pegRNAs No Xiao Wang 170132 pOsU3 Plant OsU3 pegRNA BsaI + HindIII No Caixia...using a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that...

  4. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu 45946 pU6-BbsI-chiRNA dU6 ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles, Harrison... = S. pyogenes (PAM = NGG) 49410 pCFD3-dU6:3gRNA dU6:3 BbsI Injection or in vitro transcription Virmilion...

  5. CRISPR Plasmids - C. elegans

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...transcription none, need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need...Cas9 plasmid Sternberg 177783 L4440_BioBrick-sgRNA T7 BbsI, BsaI Ingestion by C. elegans none, need Cas9...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...

  6. CRISPR Plasmids - Plants

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... pU3-gRNA OsU3 AarI none S. pyogenes Gao 53061 pZmU3-gRNA maize U3 none S. pyogenes Gao 53062 pU6-gRNA...gRNA wheat U6 BbsI none S. pyogenes Gao 59188 pBlu/gRNA Arabidopsis U6 BbsI none S. pyogenes Stupar 62200...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...

  7. CRISPR References and Information

    Type
    Collection
    ... protocol Church gRNA design and cloning gRNA cloning vector PDF 115.2 KB Church gRNA design and cloning...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF 87.2 KB Liu Fly: gRNA cloning pAc-sgRNA-Cas9...Cas9 ; pUC gRNA Shuttle PDF 488.6 KB Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF 125.9...the Chan Zuckerberg Biohub . gRNA Design Deep learning models to design gRNAs and predict their efficiency...Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized ...optimized Cas9 PDF 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo...pluripotent stem cells pCas9_GFP ; gRNA empty vector Link O'Connor-Giles Fly: gRNA and ssODNs design and cloning...
Showing: 1 - 7 of 7 results