... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function Genome...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ...virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing multiple...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single...the steps for using pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments...about each library can be found on the individual library page. Find Pooled Libraries of Interest...custom filtered list of the CRISPR pooled libraries in Addgene's collection. Library Type Activation Barcode...

... Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic...than NHEJ. Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled...CRISPR expression plasmids that have been designed for use in parasites. CRISPR... CRISPR Parasites CRISPR Plasmids...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...

...used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout, activation...preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used ...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...you need to use CRISPR in your own lab. Check out our CRISPR resources or browse plasmids below by function...Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and the basics...planning your first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've...analysis, discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...

...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...gene, so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually...For more detail on using CRISPR libraries for screens, see our pooled library guide and our blog post ...

...Cas12a plasmids Addgene's full CRISPR guide and summary of CRISPR pooled libraries . FlipGFP-based activity...useful for detection assays and more. Pooled Libraries : Pooled libraries of spike protein mutations. Research...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...SARS-CoV-2 plasmids are curated on a separate Ginkgo Bioworks Plasmid Collection page. SARS-CoV-2 Pooled Libraries...to ask them to deposit the plasmid with us. Return to top Pooled Libraries SARS-CoV-2 Spike (S) Ectodomain...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...

...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...precise DNA-targeting of CRISPR-Cas systems, allowing for the integration of large fragments of DNA at specific... or search the tables below for CRISPR Transposase plasmids. Plasmids are available for expression in ...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...

... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand DNA degradation.... or search the tables below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...

..... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts In collaboration with the labs who...deposited CRISPR plasmids , we've created a series of links and guides to help you use CRISPR in your lab... to identify potential DNA target sites for TALEs and CRISPR/Cas. CRISPRdirect From the Database Center...PDF 2.3 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9... curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community... CRISPR References and Information CRISPR References and Information You may also like...... More Guide to CRISPR technology Addgene Blog Posts How to Design Your gRNA for CRISPR Genome Editing ...

...Click here to view all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections...ORFs Synthetic Biology Pooled Libraries Pooled libraries are a set of plasmids all built with the same...curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 156,627 plasmids . We've...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...

...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas... the Doudna Lab . Browse, sort, or search the tables below for CRISPR nickase plasmids. Plasmids are available... CRISPR Nick CRISPR Plasmids: Nick...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...Nick Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying...search the tables below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR... CRISPR Purify CRISPR: Purify Genomic ...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...

...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...by the Doudna Lab . Browse, sort, or search the tables below for CRISPR RNA targeting plasmids. Plasmids...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In bacteria...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert...

...CRISPR plasmids that create double strand breaks in DNA.... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully...maintained by the Doudna Lab . Browse, sort, or search the tables below for CRISPR plasmids designed to introduce...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...

... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...

... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ... The following CRISPR plasmids have been designed for use in Xenopus. Cut Fully functional Cas9 enzymes...in the DNA, possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...maintained by the Doudna Lab . Browse, sort, or search the tables below for CRISPR plasmids for RNA editing...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR... CRISPR RNA Editing CRISPR Plasmids...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing...

...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break....CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two... by the Doudna Lab . Browse, sort, or search the tables below for CRISPR base editing plasmids. Plasmids...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...

... CRISPR Visualize CRISPR Plasmids:...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...Plasmids: Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...researchers have turned dCas9 into a customizable DNA labeler compatible with fluorescence microscopy in...

... CRISPR C. elegans CRISPR Plasmids: C. ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in C. elegans. CRISPR.... elegans Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...

...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ... The following CRISPR plasmids have been designed for use in Drosophila and other insects. Cut Fully functional...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...