... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in... viral preparation from the inventory below. Lentiviruses are distributed as purified viral particles ...non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome... (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker...

...Plasmids Lentiviral Plasmids Additional Resources: Lentivirus Guide Addgene's Viral Service Virus Biosafety...Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most...and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing ... considerations to evaluate when working with lentivirus: these viruses are based on HIV-1, which may ...Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral terms... and Packaging Plasmids One safety feature of lentivirus used in research is that the components necessary... that can be used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description...

... 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic lentiviral vector for the expression of human.... 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression of human...-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for the expression...151-9. Jaenisch Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression...polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. Jaenisch Lentivirus Mouse Doxycycline-inducible...):766-70. Nagy Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression...Delivery Method Species Description Article PI Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc...

... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core (Link ...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations...production methods and quality control for lentivirus and AAV. Viral... Viral Vector Packaging Service Viral Production...

...technologies. Penn Vector Core AAV Vectors Many AAV vectors from the Penn Vector Core have been transferred to Addgene... Viral Vector Packaging Service Addgene produces high-quality viral vector preps...and we’ll make the viral vector prep you need. Packaged on Request Viral Vector Packaging Service Information...about viral vector production at Addgene, ordering information, and more. Additional Viral Vector Resources...biosafety of viral vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from...aspects of viral vectors, including troubleshooting advice and explanations of viral vector technologies....of particular Penn Vector Core catalog items. Virus-associated DNA Each viral vector service request will...

...Biosafety Guide Blog: Plasmids 101-Viral Vector Elements Viral Vectors 101 eBook Overview of Viral Plasmids...engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses are versatile tools because of... Lab Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide...List of viral vector plasmids available at Addgene and other related resources. Special...Target Cell Genome Integration Immune Response Lentivirus Stable RNA <8 Kb 80-130 Dividing/Non-dividing...Guide University of Pennsylvania Vector Core Roth Lab Blog, "DREADD Users Blog" For providing scientific....2010.12.009. PMID: 21167871 Wold WSM, Toth K. Adenovirus vectors for gene therapy, vaccination and cancer gene therapy...

...Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral...Transfer Vectors Adenoviral Vectors Kazuhiro Oka and the Baylor College of Medicine Gene Vector Core (Link...these transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Viral...and Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and ...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of lentiviral...from the PGK promoter Adenoviral Vectors The adenoviral transfer vectors contained within this set are derived...

...shRNA cassette from another vector (e.g. pSUPER) can be cloned into this vector; Contains a 5'LTR and should...Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA Negative control vector for ...protocol for using the popular cloning vector pLKO.1. Trono Lab Lentivectors Toolbox Tyler Jacks Lab Protocols... plasmid. Below, browse our selection of empty vectors for cloning in shRNAs. Plasmids for constitutive...delivery. Constitutive Lentiviral shRNA Lentiviral vectors for constitutive shRNA expression ID Plasmid Description...Description PI 10878 pLKO.1 ‐ TRC cloning vector shRNA expression; Recommended for cloning new shRNAs ...Root 10879 pLKO.1 ‐ TRC control Negative control vector containing non-hairpin insert David Root 1864 pLKO...

...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus...Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has...

... in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP...lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression ...Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for details...oligos and clone these oligos into your desired vector. In many cases, targeting oligos are synthesized... exact cloning strategy will depend on the gRNA vector you have chosen, so it is best to review the protocol...

...Collection of adenovirus shuttle vectors and strains...Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class of medium-sized...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ...

...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore...Therefore, you must be equipped and authorized to use lentivirus in your lab. Once you have determined that CRISPR-based...TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human Stiewe N/A Varies 1,910–3,034 per exon Human...

...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual... included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...

... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...- #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with 2A...Nature 2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, ...61408 : Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling...SpCas9 or SpCas9n, and the single guide RNA. The vector can be digested using BbsI, and a pair of annealed...indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The oligos are designed...gene activation using SAM There are two sets of 3 vectors each available for mammalian endogenous gene activation...

... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors. These systems...single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids...expression vectors for insertion of custom gRNA target sequences. CRISPR... CRISPR Empty gRNA Vectors CRISPR Plasmids...Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...

...Reading Frames (ORFs) in pDONR-223 Gateway® Entry vectors from the labs of William Hahn and David Root . ...system that can be used to construct complex lentiviruses for modification of mammalian cells with a single...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building... reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope...