...Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...packaging plasmids that can be used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation...generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis...lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search...

... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled...Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in our repository... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...library expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse... of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation from the inventory...

... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available...information page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent...Method Species Description Article PI Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc, and Brd3R...Commun. 2018 Jul 6;9(1):2643. Otonkoski Lentivirus Human Expression of human Sox2, Nanog, Oct4, and Lin28...318(5858):1917-20. Thomson Lentivirus Human Doxycycline-inducible expression of human Oct4, Sox2, Klf4,...

...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...viral production methods and quality control for lentivirus and AAV. Viral...AAV Production Titer Quality Control Resources Lentivirus Production Titer Quality Control Resources Overview...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...vectors are tested in vitro and in vivo for gene expression and/or function. These data are sometimes reported...mL, or both. Two days later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was...

... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses...enabling long term transgene expression. Overview of Viral Plasmids While plasmids are typically used to encode...for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit...direct high but transient transgene expression. γ-Retrovirus Plasmids | Guide γ-retroviruses, commonly referred...organism. Figure 1. Using viral plasmids to generate virus. A) Viral plasmids have been engineered such that...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...researchers can introduce plasmids to a host to generate virus. Furthermore, these plasmids can be modified to...

...Adenoviral Guide Viral Vector Plasmids and Resources Addgene Blog: Your Lentiviral Plasmid FAQs Biosafety Resource...reporter plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing...-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1...pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre under ...pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from...promoter when FLP is expressed by another vector pCDH-EF1-Fon-tdTomato 72261 Expresses tdTomato from the ...

... shRNA expression ID Plasmid Description PI 10878 pLKO.1 ‐ TRC cloning vector shRNA expression; Recommended...shRNA expression ID Plasmid Description PI 8452 pMKO.1 puro Retroviral vector for shRNA expression Bob ...shRNA libraries Lentiviral packaging and expression plasmids Background RNA interference (RNAi) is an ...delivery of a plasmid. Below, browse our selection of empty vectors for cloning in shRNAs. Plasmids for constitutive...constitutive expression as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are...vectors for conditional (Cre-lox) shRNA expression ID Plasmid Description PI 11578 pSico Cre addition ...Lentiviral vectors for Tet-regulated shRNA expression ID Plasmid Description Tet on or off PI 11643 pLVCT-tTR-KRAB...

...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 156,344 plasmids . We've...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ... EXtracellular Plasmid RESource (EXPRESs) Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Curated Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...

...for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression of Cas9 and/or gRNA...activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR, you will need...to the transient Cas9 expression. The table below summarizes the major expression systems and variables...Delivery method Expression System Components of System Application Mammalian expression vector Cas enzyme... (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA... or electroporation Transient expression of CRISPR components Expression decreases as RNA is degraded ...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...

... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral...viruses with multicistronic expression + Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have...

...multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell. This is ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used... co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence or ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...

...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...nuclei in addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase... SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes...., Science 2013). Additionally, we have a plasmid expressing SpCas9 alone without sgRNA. Full references...nickase) + single guide RNA: These plasmids contain two expression cassettes, a human codon-optimized ...sequence. 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9...

... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...maintained by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted...polycistronic lentiviruses. Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the...the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369... sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs Drosophila Single plasmid for the expression of two...

... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional Resources Mammalian...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...