... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells... table based on plasmid name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication... 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic lentiviral vector for the expression of human.... 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression of human...-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for the expression...151-9. Jaenisch Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression...

... vector - Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA...collection of plasmids for packaging and expression. See our dedicated lentiviral plasmid page . ...pLKO.1 - TRC cloning vector - Lentiviral plasmid for mammalian gene silencing... plasmid for mammalian gene silencing pLVTHM - lentiviral plasmid for mammalian... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Background... bacterial expression vector (see tagged expression vectors from the SGC) pPro18...expression vector (see entire pPro collection ) Arrowsmith Lab Plasmids - More...

... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...genome engineering from a single lentiviral vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9...single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids...Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...time. Single plasmids containing both the gRNA and a Cas protein act as an all-in-one vector, but their ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited...

...Constitutive Lentiviral shRNA Lentiviral vectors for constitutive shRNA expression ID Plasmid Description...Cre-regulated Lentiviral shRNA Lentiviral vectors for conditional (Cre-lox) shRNA expression ID Plasmid Description...Tet-Regulated Lentiviral shRNA Lentiviral vectors for Tet-regulated shRNA expression ID Plasmid Description...for RNAi Constitutive Lentiviral Cre-regulated Lentiviral Tet-regulated Lentiviral Constitutive Retroviral...delivery of a plasmid. Below, browse our selection of empty vectors for cloning in shRNAs. Plasmids for constitutive...mouse pooled shRNA libraries Lentiviral packaging and expression plasmids Background RNA interference ...shRNA cassette from another vector (e.g. pSUPER) can be cloned into this vector; Contains a 5'LTR and should...

...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Addgene plasmid #141347) is available in standard format and as a Lentiviral prep . ID Plasmid Description...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...activity are listed below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc...Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter...

...Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral...Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and Resources...Addgene Blog: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors...series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Viral... this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've ...these transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol...

...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...virus protocols . Browse our plasmid collection of popular lentiviral vectors .... . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core (Link ...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....-free plasmid purification protocol. To minimize the immunogenic properties of the final vector preparation...

...Collections Luciferase Plasmids Luciferase Plasmid Collection You may also like... Fluorescent Proteins Blog...luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids from 2016...luciferase reporter. These plasmids improve over the original STARR-seq plasmids by using the bacterial ORI...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...regions Joshua Mendell 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...luciferase expression plasmids; these are noted with the viral service icon . ID Plasmid Luciferase Type Promoter...broad host range plasmid Attila Karsi 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of...

...Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections Background...generation lentiviral plasmid for inducible expression of shRNA; neomycin selection; plasmid 21915 has ...pInducer20 Lentiviral vector for shRNA expression; see article for additional tookit plasmids third-generation...interest tTA expression plasmid Components for a tetracycline on system are: Plasmid containing a tetracycline-dependent...selection TetR On Wiederschain 41393 pCW57.1 Lentiviral vector for inducible expression for Gateway cloning...options rtTA On Root 11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene or shRNA...or rtTA None On Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression of a miR-shRNA; selection...

... Plasmid Collections Cre-lox Plasmids Cre-lox Plasmids Additional...Cre-lox Plasmids Addgene's Blog: Cre-lox Addgene's Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs...components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre...viral preparations of plasmids that are currently in our repository. ID Plasmid Description Promoter Expression...cassette; Targeting vector for Rosa26 locus Mammalian Jacks 12238 pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 ...17408 Puro.Cre empty vector Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736 pOG231 ...Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian Luo...

...Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer ... Viral Plasmids Lentiviral Plasmids Lentiviral...of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...Looking for lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table... Also see plasmid 46948 . Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA plasmid (pGIPz based...

...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled ...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in our repository...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository ...

.../ARS vector (Leu2) that contains dCas9 fused to N 51025 pSLQ1661-sgMUC4-E3(F+E) Lentiviral vector that... CRISPR Qi Lab CRISPR Plasmids Qi Labs CRISPR Plasmids Available from... genome. 1. Two-plasmid CRISPRi system for bacterial gene knockdown The first plasmid ( pdCas9_bacteria... dcas9 gene on a p15A vector with chloramphenicol resistance. The second plasmid ( pgRNA_bacteria ) contains... both plasmids in bacteria could cause up to 300-fold repress on targeted genes. 2. Two-plasmid CRISPRi... 23849981 . Individual plasmids can be ordered via the links below: ID Plasmid Description 44246 pdCas9...fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid for use with either...

...Addgene plasmids #61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422...VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper...guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015). The dual vector system uses S. pyogenes Cas9...SpCas9 plasmid series. ), and a pair of annealed oligos can be cloned scarlessly into the vector before...

... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+...Species) Organism/Expression System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP...Verkhusha Lab Plasmids - Enhanced colors, including PSmOrange, iRFP Gradia Lab Mammalian Plasmids - Includes...Lab Plasmids - Includes many N- and C-terminal fluorescent proteins Insect Sutherland Lab Plasmids - For...expression Davidson Lab Plasmids - Includes many fluorescent proteins in the pBAD vector Jump to Top References...

...efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector or separate...stable cell lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of...Packaging and envelope plasmids provide the necessary components to make lentiviral particles (for details...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...desired vector. In many cases, targeting oligos are synthesized, annealed, and inserted into plasmids containing...particular model organism. There may not be a perfect plasmid for your specific application, and in such cases...different activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR...

...technologies. Penn Vector Core AAV Vectors Many AAV vectors from the Penn Vector Core have been transferred to Addgene...Viral Vector Packaging Service Addgene produces high-quality viral vector preps from select plasmids in ...eligible AAV plasmids and we’ll make the viral vector prep you need. Packaged on Request Viral Vector Packaging.... We know that viral vector production is a common step after requesting plasmids from Addgene, and by...In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer preps...about viral vector production at Addgene, ordering information, and more. Additional Viral Vector Resources...biosafety of viral vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from...

...scheme Fujii Lab Plasmids Individual plasmids can be ordered via the links below: ID Plasmid Description 47948...elements/pMD20 iChIP plasmid, LexA-binding elements 48874 3xFNLDD/pCMV-7.1 iChIP plasmid, expresses 3xFNLDD...-I2 iChIP plasmid, expresses 3xFNLDD in mammalian cells 48972 3xFNLDD/pMXs-IG iChIP plasmid, expresses...interest 85586 gRNA Cloning Vector Bbs I ver. 2 An empty sgRNA expression vector. sgRNA sequence can be cloned...ver. 2 structure. 92220 pLenti_dCas9-2xAM Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in ...Bbs I sites. 92221 pLenti_dCas9-2xAM_hIRF-1 Lentiviral plasmid expressing dCas9-2xAM and gRNA for human ... gRNA plasmids from Dr. Hodaka Fujii's lab. Genome...

... of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR... CRISPR Plasmids and Resources...inhibition, and barcoding experiments. Browse CRISPR Plasmids by Function Genome Engineering Cut Wild-type Cas...variety of factors. gRNAs gRNA plasmids to target specific genes....

...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have...